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首页> 外文期刊>Acta biomaterialia >In vitro responses to electrosprayed alkaline phosphatase/calcium phosphate composite coatings.
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In vitro responses to electrosprayed alkaline phosphatase/calcium phosphate composite coatings.

机译：对电喷涂碱性磷酸酶/磷酸钙复合涂层的体外反应。

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摘要

Surface modification of titanium implants to improve their fixation in bone tissue is of great interest. We present a novel approach to enhance implant performance by applying important principles of bone mineralization to biomedical coatings. As an attempt to mimic the biphasic biomineralization process, both the enzyme alkaline phosphatase (ALP) and calcium phosphate (CaP) were immobilized onto Ti discs, thereby triggering enzymatically and physicochemically controlled biomineralization pathways. ALP, CaP and ALP-CaP composite coatings with preserved functionality of ALP were successfully deposited using electrospray deposition. In vitro soaking studies in cell culture medium revealed that crystal growth initially proceeded at a faster rate on CaP-coated Ti than on ALP-containing coatings, but mineral deposition onto ALP-coated Ti caught up with the calcification behaviour of CaP coatings upon long-term soaking. Cell culture experiments with osteoblast-like cells, however, demonstrated the opposite effect in mineral deposition on the electrosprayed CaP and ALP coatings. The ALP-CaP composite coatings showed delayed proliferation as well as accelerated mineralization in comparison to cells cultured on the CaP-coated and uncoated Ti. In conclusion, these in vitro results showed that the osteogenic potential of Ti can be stimulated by ALP-containing coatings.
机译：钛植入物的表面改性以改善其在骨组织中的固定是非常令人感兴趣的。我们提出了一种通过将骨矿化的重要原理应用于生物医学涂层来提高植入物性能的新颖方法。为了模仿双相生物矿化过程，碱性磷酸酶（ALP）和磷酸钙（CaP）均被固定在Ti盘上，从而触发了酶法和物理化学控制的生物矿化途径。使用电喷雾沉积成功地沉积了具有ALP保留功能的ALP，CaP和ALP-CaP复合涂层。在细胞培养基中进行的体外浸泡研究表明，与不含ALP的涂层相比，CaP涂层的Ti最初的晶体生长速度更快，但是长期沉积后，矿物沉积到ALP涂层的Ti上的钙化行为赶上了CaP涂层的钙化行为。长期浸泡。然而，用成骨细胞样细胞进行的细胞培养实验表明，在电喷雾的CaP和ALP涂层上，矿物质沉积具有相反的作用。与在CaP涂层和未涂层​​的Ti上培养的细胞相比，ALP-CaP复合涂层显示出延迟的增殖以及加速的矿化作用。总之，这些体外结果表明，含ALP涂层可以激发Ti的成骨潜能。

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