• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Alpha,beta-poly(L-aspartate-graft-PEI): A pseudo-branched PEI as a gene carrier with low toxicity and high transfection efficiency.
【24h】

Alpha,beta-poly(L-aspartate-graft-PEI): A pseudo-branched PEI as a gene carrier with low toxicity and high transfection efficiency.

机译：α，β-聚（L-天冬氨酸-移植物-PEI）：伪支链PEI作为基因载体，具有低毒性和高转染效率。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this research is to develop a novel branched polyethylenimine (PEI)-like polycation as a potential gene carrier with high gene transfection efficiency and low toxicity. In particular, alpha,beta-poly(l-aspartate-graft-PEI) (Asp-g-PEI), a pseudo-branched PEI, was synthesized by the ring-opening reaction of poly(l-succinimide) (PSI) with low molecular weight branched PEI (LMW PEI, MW=600 and 1200). Good plasmid condensation and protection ability of Asp-g-PEI were confirmed by agarose gel electrophoresis assay. Asp-g-PEI/DNA complexes showed high positive zeta potential, narrow size distribution, good dispersity and a compact spherical shape with size below 250nm when the N/P ratio was above 5, suggesting that they can be endocytosed. Cytotoxicity of Asp-g-PEI/DNA complexes was rather lower than that of PEI25K/DNA complexes, especially at high N/P ratio. The most efficient gene transfection of Asp-g-PEI/DNA complexes was similar or a little higher than that of PEI25K in 293T, HeLa and HepG2 cell lines, while almost 4 and 6 times higher than that of parent PEI1200 and PEI600, respectively, in HeLa cell line; as the molecular weight of parent PEI in Asp-g-PEI was increased from 600 to 1200, the transfection efficiency showed a tendency to decrease. The mechanism of Asp-g-PEI-mediated gene transfection was attributed to the "proton sponge effect" due to PEI in the copolymer.
机译：这项研究的目的是开发一种新型的支链聚乙烯亚胺（PEI）样聚阳离子作为具有高基因转染效率和低毒性的潜在基因载体。特别地，通过聚（1-琥珀酰亚胺）（PSI）与聚（1-琥珀酰亚胺）的开环反应合成了α，β-聚（1-天冬氨酸-接枝-PEI）（Asp-g-PEI），一种伪支链PEI。低分子量支链PEI（LMW PEI，MW = 600和1200）。琼脂糖凝胶电泳证实了Asp-g-PEI具有良好的质粒缩合和保护能力。当N / P大于5时，Asp-g-PEI / DNA复合物显示出较高的正Zeta电位，窄的粒度分布，良好的分散性和尺寸小于250nm的紧凑球形，表明它们可以被内吞。 Asp-g-PEI / DNA复合物的细胞毒性要比PEI25K / DNA复合物低，尤其是在高N / P比的情况下。在293T，HeLa和HepG2细胞系中，Asp-g-PEI / DNA复合物最有效的基因转染相似或略高于PEI25K，而分别比亲本PEI1200和PEI600高近4和6倍，在HeLa细胞系中;随着Asp-g-PEI中母体PEI的分子量从600增加到1200，转染效率呈下降趋势。 Asp-g-PEI介导的基因转染的机制归因于共聚物中的PEI引起的“质子海绵效应”。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号