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首页> 外文期刊>Acta biomaterialia >Specific interactions between human fibroblasts and particular chondroitin sulfate molecules for wound healing.
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Specific interactions between human fibroblasts and particular chondroitin sulfate molecules for wound healing.

机译：人成纤维细胞与特定硫酸软骨素分子之间的特异性相互作用，可促进伤口愈合。

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摘要

The chondroitin sulfates (CSs) constitute an important group of biomacromolecules in the extracellular matrix. However, limited information is available about their specific biological functions. This study aimed to define the interactions between cells and various types of CS. The effects of CSs on cellular activities and the cell cycle were evaluated using cell culture, RNA interference, real-time polymerase chain reaction, flow cytometry, wound healing and contraction models. The results showed that C-6-S promoted both cell proliferation and adhesion, while C-4-S promoted proliferation but inhibited adhesion. Moreover, knockdown of chondroitin inhibited cell proliferation and migration, as well as arresting cells in the G(2)/M phase. Also, both C-4-S and C-6-S promoted wound closure in a two-dimensional wound model, whereas only C-6-S inhibited wound contraction in a three-dimensional wound model. This study illustrates that the interaction between cells and different CSs are specific and sulfate-group-dependent. These findings provide useful information for better applications of CSs for wound healing.
机译：硫酸软骨素（CSs）在细胞外基质中构成了重要的生物大分子组。但是，有关其特定生物学功能的信息有限。这项研究旨在定义细胞和各种类型的CS之间的相互作用。使用细胞培养，RNA干扰，实时聚合酶链反应，流式细胞仪，伤口愈合和收缩模型评估CS对细胞活性和细胞周期的影响。结果表明，C-6-S促进细胞增殖和粘附，而C-4-S促进增殖但抑制粘附。此外，软骨素的基因抑制抑制细胞增殖和迁移，以及将细胞停在G（2）/ M期。同样，在二维伤口模型中，C-4-S和C-6-S都促进伤口闭合，而在三维伤口模型中，只有C-6-S抑制伤口收缩。这项研究表明，细胞与不同CS之间的相互作用是特异性的，并且是硫酸盐基团依赖性的。这些发现为CS在伤口愈合中的更好应用提供了有用的信息。

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