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首页> 外文期刊>Acta biomaterialia >Osteogenic differentiation of bone marrow stromal cells on poly(epsilon-caprolactone) nanofiber scaffolds.
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Osteogenic differentiation of bone marrow stromal cells on poly(epsilon-caprolactone) nanofiber scaffolds.

机译：聚（ε-己内酯）纳米纤维支架上骨髓基质细胞的成骨分化。

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摘要

Nanofiber poly(epsilon-caprolactone) (PCL) scaffolds were fabricated by electrospinning, and their ability to enhance the osteoblastic behavior of marrow stromal cells (MSCs) in osteogenic media was investigated. MSCs were isolated from Wistar rats and cultured on nanofiber scaffolds to assess short-term cytocompatibility and long-term phenotypic behavior. Smooth PCL substrates were used as control surfaces. The short-term cytocompatibility results indicated that nanofiber scaffolds supported greater cell adhesion and viability compared with control surfaces. In osteogenic conditions, MSCs cultured on nanofiber scaffolds also displayed increased levels of alkaline phosphatase activity for 3 weeks of culture. Calcium phosphate mineralization was substantially accelerated on nanofiber scaffolds compared to control surfaces as indicated through von Kossa and calcium staining, scanning electron microscopy and energy-dispersive X-ray spectroscopy. Increased levels of intra- and extracellular levels of osteocalcin and osteopontin were observed on nanofiber scaffolds using immunofluorescence techniques after 3 weeks of culture. These results demonstrate the enhanced tissue regeneration property of nanofiber scaffolds, which may be of potential use for engineering osteogenic scaffolds for orthopedic applications.
机译：纳米纤维聚（ε-己内酯）（PCL）支架通过静电纺丝制造，并研究了它们在成骨培养基中增强骨髓基质细胞（MSCs）成骨行为的能力。从Wistar大鼠中分离出MSC，并在纳米纤维支架上培养以评估短期细胞相容性和长期表型行为。光滑的PCL基板用作控制面。短期细胞相容性结果表明，与对照表面相比，纳米纤维支架支持更大的细胞粘附性和活力。在成骨条件下，在纳米纤维支架上培养的MSC在培养3周后还表现出增加的碱性磷酸酶活性。如通过冯·科萨（von Kossa）和钙染色，扫描电子显微镜和能量色散X射线光谱法所表明的，与对照表面相比，纳米纤维支架上的磷酸钙矿化作用显着加速。培养3周后，使用免疫荧光技术在纳米纤维支架上观察到骨钙素和骨桥蛋白的细胞内和细胞外水平升高。这些结果证明了纳米纤维支架的增强的组织再生特性，其可能潜在地用于工程骨科支架的成骨支架。

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