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首页> 外文期刊>Acta biomaterialia >Statistical approach in alginate membrane formulation for cell encapsulation in a GMP-based cell factory.
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Statistical approach in alginate membrane formulation for cell encapsulation in a GMP-based cell factory.

机译：基于GMP的细胞工厂中用于细胞封装的藻酸盐膜制剂的统计方法。

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A cell encapsulation technology in alginate has been developed with the aim of obtaining cell controlled release or three-dimensional cultures. The aim of this work is to verify the predictability of alginate capsules for large-scale production by Good Manufacturing Practice (GMP) standardized procedures in a cell factory. A cell-free capsule model was performed following the GMP guidelines: an opaque agent suspension in a bivalent cation solution (Ca(2+), Ba(2+), Sr(2+)) was dropped in a sodium alginate solution, obtaining capsules presenting a liquid core surrounded by a gel alginate membrane. The concentration of the ion, and the treatment with protamine, can considerably vary the characteristics of the capsules (weight, whole diameter, core diameter, gel capsule thickness, capsule strength). It is therefore possible to optimize the performance of the capsules, relating the molecular structure and size of the polymeric membrane to the desired functional properties. Technological resources are available for large-scale cell encapsulation intended for advanced therapies (gene therapy, somatic cell therapy and tissue engineering) in a cell factory, following GMP guidelines.
机译：为了获得细胞控释或三维培养物，已经开发了藻酸盐中的细胞包封技术。这项工作的目的是通过细胞工厂中的良好生产规范（GMP）标准化程序来验证藻酸盐胶囊用于大规模生产的可预测性。按照GMP指南进行无细胞胶囊模型的制备：将不透明的试剂悬浮在二价阳离子溶液（Ca（2 +），Ba（2 +），Sr（2+））中，然后放入海藻酸钠溶液中，得到胶囊具有被藻酸盐凝胶膜包围的液芯。离子的浓度以及鱼精蛋白的处理会大大改变胶囊的特性（重量，整个直径，核心直径，凝胶胶囊厚度，胶囊强度）。因此有可能优化胶囊的性能，使聚合物膜的分子结构和尺寸与所需的功能性质相关。遵循GMP准则，可为细胞工厂中的先进疗法（基因疗法，体细胞疗法和组织工程）提供用于大规模细胞封装的技术资源。

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