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首页> 外文期刊>Acta biomaterialia >Increased mucociliary differentiation of human respiratory epithelial cells on hyaluronan-derivative membranes.
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Increased mucociliary differentiation of human respiratory epithelial cells on hyaluronan-derivative membranes.

机译：透明质酸衍生物膜上人类呼吸道上皮细胞的粘膜纤毛分化增加。

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摘要

The selection of a scaffold to facilitate mucociliary differentiation of respiratory epithelial cells (RECs) is crucial in the development of tissue engineering of respiratory epithelium. However, how the differentiation of RECs is influenced by the biomaterials has never been thoroughly explored. Previously, hyaluronan derivatives were considered as unsuitable biomaterials for culture of respiratory epithelium. In contrast, this study demonstrates that the membranous scaffolds made from benzyl esters of hyaluronic acids are capable of providing a more preferential environment for human RECs than conventionally used collagen-based scaffolds. The proliferation and mucociliary differentiation of RECs were examined by MTT assays, scanning electron microscopy, immunofluorescence, immunoblotting and gene expression. The percentage of ciliated cells in cultured RECs increased from 12.4% on collagen to 20.4% on hyaluronan-derivative membranes with a pseudostratified polarized layer that closely resembled the composition of the native epithelium. The expression levels of MUC5AC and MUC5B mRNA were higher on hyaluronan-based scaffolds than those on collagen. The presence of a hyaluronan-binding domain, CD44 and the receptor for hyaluronan-mediated motility of RECs were also demonstrated. Accordingly, the mucociliary differentiation-promoting effect of hyaluronan-derivative membranes indicates that it may be applied to the tissue engineering of respiratory epithelium.
机译：选择支架以促进呼吸道上皮细胞（RECs）的粘膜纤毛分化对于呼吸道上皮组织工程的发展至关重要。但是，尚未彻底探讨RECs的分化如何受到生物材料的影响。以前，透明质酸衍生物被认为是不适合培养呼吸道上皮的生物材料。相反，该研究表明，由透明质酸的苄基酯制成的膜状支架比常规使用的基于胶原的支架能够为人类REC提供更优越的环境。通过MTT测定，扫描电子显微镜，免疫荧光，免疫印迹和基因表达检查了REC的增殖和粘膜纤毛分化。培养的REC中的纤毛细胞百分比从胶原蛋白上的12.4％增加到透明质酸衍生物膜上的20.4％，透明质酸膜上的假层化偏振层与天然上皮的组成非常相似。在透明质酸基支架上，MUC5AC和MUC5B mRNA的表达水平高于胶原蛋白。还证实了透明质酸结合结构域CD44和透明质酸介导的RECs的受体的存在。因此，透明质酸衍生物膜的粘膜纤毛分化促进作用表明它可以应用于呼吸道上皮的组织工程。

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