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首页> 外文期刊>Acta biomaterialia >Repair of osteochondral defects with biodegradable hydrogel composites encapsulating marrow mesenchymal stem cells in a rabbit model.
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Repair of osteochondral defects with biodegradable hydrogel composites encapsulating marrow mesenchymal stem cells in a rabbit model.

机译：用可生物降解的水凝胶复合材料包裹兔骨髓间充质干细胞修复骨软骨缺损。

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This work investigated the delivery of marrow mesenchymal stem cells (MSCs), with or without the growth factor transforming growth factor-beta1 (TGF-beta1), from biodegradable hydrogel composites on the repair of osteochondral defects in a rabbit model. Three formulations of oligo(poly(ethylene glycol) fumarate) (OPF) hydrogel composites containing gelatin microparticles (GMPs) and MSCs were implanted in osteochondral defects, including (i) OPF/GMP hydrogel composites; (ii) OPF/GMP hydrogel composites encapsulating MSCs; and (iii) OPF hydrogel composites containing TGF-beta1-loaded GMPs and MSCs. At 12weeks, the quality of new tissue formed in chondral and subchondral regions of defects was evaluated based on subjective and quantitative histological analysis. OPF hydrogel composites were partially degraded and the defects were filled with newly formed tissue at 12weeks with no sign of persistent inflammation. With the implantation of scaffolds alone, newly formed chondral tissue had an appearance of hyaline cartilage with zonal organization and intense staining for glycosaminoglycans, while in the subchondral region hypertrophic cartilage with some extent of bone formation was often observed. The addition of MSCs, especially with TGF-beta1-loaded GMPs, facilitated subchondral bone formation, as evidenced by more trabecular bone appearance. However, the delivery of MSCs with or without TGF-beta1 at the dosage investigated did not improve cartilage morphology. While OPF-based hydrogel composites supported osteochondral tissue generation, further investigations are necessary to elucidate the effects of MSC seeding density and differentiation stage on new tissue formation and regeneration.
机译：这项工作研究了从可生物降解的水凝胶复合物在兔模型中骨软骨缺损修复中的骨髓间充质干细胞（MSCs）的输送，无论是否具有生长因子转化生长因子-beta1（TGF-beta1）。将三种含有明胶微粒（GMP）和MSC的低聚（聚（富马酸乙二醇））（OPF）水凝胶复合物植入骨软骨缺损中，包括（i）OPF / GMP水凝胶复合物; （ii）包裹MSC的OPF / GMP水凝胶复合材料; （iii）包含TGF-β1的GMP和MSC的OPF水凝胶复合物。在第12周时，根据主观和定量组织学分析评估在软骨缺损和软骨下区域形成的新组织的质量。 OPF水凝胶复合材料在12周时会部分降解，缺损处会充满新形成的组织，没有持续性炎症的迹象。仅通过植入支架，新形成的软骨组织就出现了透明的软骨，具有带状组织和强的糖胺聚糖染色，而在软骨下区域，经常观察到肥大性软骨并具有一定程度的骨形成。 MSC的添加，特别是TGF-β1加载的GMP的添加，促进了软骨下骨的形成，更多的小梁骨外观证明了这一点。但是，以所研究的剂量递送含或不含TGF-β1的MSC并不能改善软骨形态。尽管基于OPF的水凝胶复合材料支持骨软骨组织的生成，但有必要进行进一步的研究以阐明MSC接种密度和分化阶段对新组织形成和再生的影响。

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