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首页> 外文期刊>Acta biomaterialia >Covalently immobilized RGD gradient on PEG hydrogel scaffold influences cell migration parameters.
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Covalently immobilized RGD gradient on PEG hydrogel scaffold influences cell migration parameters.

机译：PEG水凝胶支架上共价固定的RGD梯度会影响细胞迁移参数。

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摘要

Understanding the influence of a controlled spatial distribution of biological cues on cell activities can be useful to design "cell instructive" materials, able to control and guide the formation of engineered tissues in vivo and in vitro. To this purpose, biochemical and mechanical properties of the resulting biomaterial must be carefully designed and controlled. In this work, the effect of covalently immobilized RGD peptide gradients on poly(ethylene glycol) diacrylate hydrogels on cell behaviour was studied. We set up a mechanical device generating gradients based on a fluidic chamber. Cell response to RGD gradients with different slope (0.7, 1 and 2 mM cm(-1)) was qualitatively and quantitatively assessed by evaluating cell adhesion and, in particular, cell migration, compared to cells seeded on hydrogels with uniform distribution of RGD peptides. To evaluate the influence of RGD gradient and to exclude any concentration effect on cell response, all analyses were carried out in a specific region of the gradients which displayed the same average concentration of RGD (1.5 mM). Results suggest that cells recognize the RGD gradient and adhere onto it assuming a stretched shape. Moreover, cells tend to migrate in the direction of the gradient, as their speed is higher than that of cells migrating on hydrogels with a uniform distribution of RGD and increases by increasing RGD gradient steepness. This increment is due to an augmentation of bias speed component of the mean squared speed, that is, the drift of the cell population migrating on the anisotropic surface provided by the RGD gradient.
机译：了解生物线索的受控空间分布对细胞活性的影响可能对设计“细胞指导”材料有用，该材料能够在体内和体外控制和指导工程组织的形成。为此，必须仔细设计和控制所得生物材料的生物化学和机械性能。在这项工作中，研究了共价固定的RGD肽梯度对聚（乙二醇）二丙烯酸酯水凝胶对细胞行为的影响。我们建立了一个基于射流室产生梯度的机械装置。通过评估细胞粘附力，特别是细胞迁移，定性和定量评估了对具有不同斜率（0.7、1和2 mM cm（-1））的RGD梯度的细胞反应，与接种在具有RGD肽均匀分布的水凝胶上的细胞相比。为了评估RGD梯度的影响并排除任何浓度对细胞反应的影响，所有分析均在梯度的特定区域内进行，该区域显示出相同的RGD平均浓度（1.5 mM）。结果表明细胞可以识别RGD梯度并以拉伸形状粘附在其上。而且，细胞趋向于沿梯度方向迁移，因为它们的速度高于具有均匀分布的RGD的水凝胶上迁移的细胞的速度，并通过增加RGD梯度陡度而增加。该增加是由于均方速度的偏置速度分量的增加，即，由RGD梯度提供的在各向异性表面上迁移的细胞群体的漂移。

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