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首页> 外文期刊>Acta biomaterialia >Tuning cell adhesion by controlling the roughness and wettability of 3D microano silicon structures.
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Tuning cell adhesion by controlling the roughness and wettability of 3D microano silicon structures.

机译：通过控制3D微米/纳米硅结构的粗糙度和润湿性来调整细胞粘附。

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摘要

The aim of this study is to investigate fibroblast cell adhesion and viability on highly rough three-dimensional (3D) silicon (Si) surfaces with gradient roughness ratios and wettabilities. Culture surfaces were produced by femtosecond (fs) laser structuring of Si wafers and comprised forests of conical spikes exhibiting controlled dual-scale roughness at both the micro- and the nano-scale. Variable roughness could be achieved by changing the laser pulse fluence and control over wettability and therefore surface energy could be obtained by covering the structures with various conformal coatings, which altered the surface chemistry without, however, affecting morphology. The results showed that optimal cell adhesion was obtained for small roughness ratios, independently of the surface wettability and chemistry, indicating a non-monotonic dependence of fibroblast adhesion on surface energy. Additionally, it was shown that, for the same degree of roughness, a proper change in surface energy could switch the behaviour from cell-phobic to cell-philic and vice versa, transition that was always correlated to surface wettability. These experimental findings are discussed on the basis of previous theoretical models describing the relation of cell response to surface energy. The potential use of the patterned Si substrates as model scaffolds for the systematic exploration of the role of 3D microano morphology and/or surface energy on cell adhesion and growth is envisaged.
机译：这项研究的目的是研究具有梯度粗糙度比和润湿性的高度粗糙的三维（3D）硅（Si）表面上的成纤维细胞粘附和活力。培养表面是通过飞秒（fs）激光对硅片进行结构化处理而制成的，其中包括圆锥形针刺林，在微米和纳米级均显示出受控的双尺度粗糙度。可变的粗糙度可以通过改变激光脉冲通量和控制润湿性来实现，因此可以通过用各种保形涂层覆盖结构来获得表面能，这改变了表面化学性质，但是不影响形态。结果表明，对于低粗糙度比，可获得最佳的细胞粘附力，而与表面润湿性和化学性质无关，这表明成纤维细胞粘附力对表面能的非单调依赖性。另外，还表明，对于相同程度的粗糙度，适当改变表面能可以将行为从疏孔性切换为亲疏性，反之亦然，而转变始终与表面润湿性相关。这些实验结果是在先前描述细胞反应与表面能的关系的理论模型的基础上讨论的。设想将图案化的Si衬底用作模型支架的潜在用途，用于系统探索3D微/纳米形态和/或表面能对细胞粘附和生长的作用。

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