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首页> 外文期刊>Acta biomaterialia >Multi-functional P(3HB) microsphere/45S5 Bioglass-based composite scaffolds for bone tissue engineering.
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Multi-functional P(3HB) microsphere/45S5 Bioglass-based composite scaffolds for bone tissue engineering.

机译：多功能P（3HB）微球/ 45S5生物玻璃基复合支架，用于骨组织工程。

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Novel multi-functional P(3HB) microsphere/45S5 Bioglass-based composite scaffolds exhibiting potential for drug delivery were developed for bone tissue engineering. 45S5 Bioglass-based glass-ceramic scaffolds of high interconnected porosity produced using the foam-replication technique were coated with biodegradable microspheres (size<2 microm) made from poly(3-hydroxybutyrate), P(3HB), produced using Bacillus cereus SPV. A solid-in-oil-in-water emulsion solvent extraction/evaporation technique was used to produce these P(3HB) microspheres. A simple slurry-dipping method, using a 1 wt.% suspension of P(3HB) microspheres in water, dispersed by an ultrasonic bath, was used to coat the scaffold, producing a uniform microsphere coating throughout the three-dimensional scaffold structure. Compressive strength tests confirmed that the microsphere coating slightly enhanced the scaffold mechanical strength. It was also confirmed that the microsphere coating did not inhibit the bioactivity of the scaffold when immersed in simulated body fluid (SBF) for up to 4 weeks. The hydroxyapatite (HA) growth rate on P(3HB) microsphere-coated 45S5 Bioglass composite scaffolds was very similar to that on the uncoated control sample, qualitatively indicating similar bioactivity. However, the surface topography of the HA surface layer was affected as shown by results obtained from white light interferometry. The roughness of the surface was much higher for the P(3HB) microsphere-coated scaffolds than for the uncoated samples, after 7 days in SBF. This feature would facilitate cell attachment and proliferation. Finally, gentamycin was successfully encapsulated into the P(3HB) microspheres to demonstrate the drug delivery capability of the scaffolds. Gentamycin release kinetics was determined using liquid chromatography-mass spectrometry. The release of the drug from the coated composite scaffolds was slow and controlled when compared to the observed fast and relatively uncontrolled drug release from the bone scaffold (without microsphere coating). Thus, this unique multifunctional bioactive composite scaffold has the potential to enhance cell attachment and to provide controlled delivery of relevant drugs for bone tissue engineering.
机译：新型的多功能P（3HB）微球/ 45S5生物玻璃基复合材料支架展示了药物输送的潜力，被开发用于骨组织工程。使用泡沫复制技术生产的高互连孔隙度的45S5生物玻璃基玻璃陶瓷支架涂有可生物降解的微球体（尺寸<2微米），该微球体由蜡状芽孢杆菌SPV生产的聚（3-羟基丁酸酯）P（3HB）制成。使用水包油固体乳液溶剂萃取/蒸发技术生产这些P（3HB）微球。一种简单的浆料浸渍方法，使用通过超声浴分散的1％（重量）的P（3HB）微球在水中的悬浮液来涂覆支架，从而在整个三维支架结构上产生均匀的微球涂层。抗压强度测试证实了微球涂层稍微增强了支架的机械强度。还证实了，当将微球涂层浸入模拟体液（SBF）中长达4周时，其不会抑制支架的生物活性。 P（3HB）微球涂层45S5生物玻璃复合支架上的羟基磷灰石（HA）生长速率与未涂覆的对照样品上的羟基磷灰石（HA）生长速率非常相似，定性表明了相似的生物活性。然而，如从白光干涉法获得的结果所示，HA表面层的表面形貌受到影响。在SBF中放置7天后，P（3HB）微球涂层支架的表面粗糙度比未涂层样品的表面粗糙度高得多。该特征将促进细胞附着和增殖。最后，庆大霉素被成功封装到P（3HB）微球中，以证明支架的药物传递能力。使用液相色谱-质谱法测定庆大霉素的释放动力学。与观察到的从骨支架（无微球涂层）的快速且相对不受控制的药物释放相比，药物从涂覆的复合支架中的释放缓慢且受控。因此，这种独特的多功能生物活性复合支架具有增强细胞附着并为骨组织工程提供相关药物的受控递送的潜力。

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