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首页> 外文期刊>Acta biomaterialia >Encapsulation of fibroblasts causes accelerated alginate hydrogel degradation.
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Encapsulation of fibroblasts causes accelerated alginate hydrogel degradation.

机译：成纤维细胞的包囊导致藻酸盐水凝胶加速降解。

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摘要

Calcium-alginate hydrogel has been widely studied as a material for cell encapsulation for tissue engineering. At present, the effect that cells have on the degradation of alginate hydrogel is largely unknown. We have shown that fibroblasts encapsulated at a density of 7.5 x 10(5) cells ml(-1) in both 2% and 5% w/v alginate remain viable for at least 60 days. Rheological analysis was used to study how the mechanical properties exhibited by alginate hydrogel changed during 28 days in vitro culture. Alginate degradation was shown to occur throughout the study but was greatest within the first 7 days of culture for all samples, which correlated with a sharp release of calcium ions from the construct. Fibroblasts were shown to increase the rate of degradation during the first 7 days when compared with acellular samples in both 2% and 5% w/v gels, but after 28 days both acellular and cell-encapsulating samples retained disc-shaped morphologies and gel-like spectra. The results demonstrate that although at an early stage cells influence the mechanical properties of encapsulating alginate, over a longer period of culture, the hydrogels retain sufficient mechanical integrity to exhibit gel-like properties. This allows sustained immobilization of the cells at the desired location in vivo where they can produce extracellular matrix and growth factors to expedite the healing process.
机译：海藻酸钙水凝胶已被广泛研究为组织工程细胞封装的材料。目前，细胞对藻酸盐水凝胶降解的影响尚不清楚。我们已经表明，在2％和5％w / v藻酸盐中以7.5 x 10（5）细胞ml（-1）密度封装的成纤维细胞至少可存活60天。流变学分析用于研究藻酸盐水凝胶在体外培养28天后所表现出的机械性能如何变化。在整个研究中均显示藻酸盐降解发生，但对于所有样品而言，在培养的前7天中藻酸盐降解最大，这与从构建物中钙离子的急剧释放有关。与2％和5％w / v凝胶中的无细胞样品相比，成纤维细胞在头7天内显示出增加的降解速率，但28天后，无细胞和包囊细胞的样品均保持了圆盘状形态和凝胶状态。像光谱。结果表明，尽管在早期阶段细胞会影响藻酸盐包封的机械性能，但在更长的培养时间内，水凝胶保留了足够的机械完整性以呈现凝胶状特性。这允许将细胞持续固定在体内所需的位置，在此处它们可以产生细胞外基质和生长因子以加快愈合过程。

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