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首页> 外文期刊>Acta biomaterialia >Fetal bovine serum xenoproteins modulate human monocyte adhesion and protein release on biomaterials in vitro.
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Fetal bovine serum xenoproteins modulate human monocyte adhesion and protein release on biomaterials in vitro.

机译：胎牛血清异种蛋白在体外调节人单核细胞粘附和蛋白质在生物材料上的释放。

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Monocyte-derived macrophages are critical in the host-foreign body response to biomaterials and have been studied extensively in various culture conditions in vitro, such as medium supplemented with fetal bovine serum (FBS) or autologous human serum (AHS). Since monocyte maturation into macrophages is highly plastic and may vary considerably depending on the surface, isolation procedures and in vitro culture conditions, we hypothesize that variations in protein adsorption and serum type will greatly impact monocyte behavior in a surface-dependent manner. The impact of xenoproteins on monocyte-surface interactions has not been well studied methodically and the use of AHS rather than FBS for macrophage-biomaterials studies in vitro is far from universal. The commonly used reference materials - tissue culture polystyrene (TCPS), polyethylene glycol (PEG) and polydimethylsiloxane (PDMS) - were employed in this study and we found a 3-fold higher adherent monocyte density on TCPS when AHS was used vs. FBS-supplemented medium. On PEG hydrogels, an 8- to 10-fold higher adhesion density was observed when AHS was employed vs. FBS, while on PDMS no difference in adhesion density was observed between the two sera conditions. Additionally, the presence of lipopolysaccharide abrogated the serum-dependent effect on cell adhesion on TCPS. Significantly different variations in protein release were observed between the serum conditions on these surfaces; in particular, there was a 100-fold higher concentration of growth-related oncogene for the AHS condition on PDMS even though the adhesion levels were comparable between the two serum conditions. These results emphasize the combined impact of the surface type and FBS xenoproteins in mediating the observed monocyte response to biomaterials in vitro.
机译：单核细胞衍生的巨噬细胞在宿主对生物材料的体外反应中至关重要，并且已在各种体外培养条件下进行了广泛研究，例如补充胎牛血清（FBS）或自体人类血清（AHS）的培养基。由于单核细胞成熟为巨噬细胞是高度可塑性的，并且可能取决于表面，分离程序和体外培养条件而有很大差异，因此我们假设蛋白质吸附和血清类型的变化将以表面依赖性方式极大地影响单核细胞的行为。异种蛋白对单核细胞-表面相互作用的影响尚未得到系统的深入研究，在体外进行巨噬细胞生物材料研究时，使用AHS而非FBS的研究还远未普及。在这项研究中使用了常用的参考材料-组织培养聚苯乙烯（TCPS），聚乙二醇（PEG）和聚二甲基硅氧烷（PDMS）-我们发现，使用AHS时，TCPS上粘附的单核细胞密度比FBS-高3倍。补充培养基。在PEG水凝胶上，与ABS相比，使用AHS时，粘附密度提高了8到10倍，而在PDMS上，两种血清条件之间的粘附密度没有差异。另外，脂多糖的存在消除了血清对TCPS细胞粘附的依赖性。在这些表面上的血清条件之间观察到蛋白质释放的显着不同变化。特别是，PDHS上AHS条件的生长相关致癌基因浓度高100倍，即使两种血清条件之间的粘附水平相当。这些结果强调了表面类型和FBS异种蛋白在介导体外观察到的单核细胞对生物材料反应的综合影响。

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