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首页> 外文期刊>Acta biomaterialia >Effects of electrospun submicron fibers in calcium phosphate cement scaffold on mechanical properties and osteogenic differentiation of umbilical cord stem cells.
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Effects of electrospun submicron fibers in calcium phosphate cement scaffold on mechanical properties and osteogenic differentiation of umbilical cord stem cells.

机译：磷酸钙水泥支架中的电纺亚微米纤维对脐带干细胞力学性能和成骨分化的影响。

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摘要

Fibrous scaffolds are promising for tissue engineering because of the high surface area and fibrous features mimicking the extracellular matrix in vivo. Calcium phosphate cements (CPCs) can be injected and self-set in the bone defect. A literature search revealed that there have been no reports on stem cell seeding on CPC containing electrospun submicron fibers. The objective of this study was to investigate for the first time the effects of electrospun fibers in CPC on mechanical properties and human umbilical cord mesenchymal stem cell (hUCMSC) proliferation, osteogenic differentiation and mineralization. Poly(D,L-lactide-co-glycolide) fibers were made via an electrospinning technique to yield an average fiber diameter of 650 nm. The fibers were incorporated into CPC consisting of tetracalcium phosphate, dicalcium phosphate anhydrous and chitosan lactate. Fiber volume fractions were 0%, 2.5%, 5% and 10%. CPC with 10% fibers had a flexural strength that was twice that of CPC without fibers, and a work-of-fracture (toughness) that was an order of magnitude larger than that of CPC without fibers. hUCMSCs proliferated rapidly and synthesized bone minerals when attached to the electrospun fiber-CPC scaffolds. Alkaline phosphatase, osteocalcin and collagen I expressions of hUCMSCs were doubled, while mineralization was increased by 40%, when fiber volume fraction in CPC was increased from 0% to 10%. The enhanced cell function was attributed to the high surface area and biomimetic features of the fiber-CPC scaffold. In conclusion, incorporating submicron fibers into CPC greatly improved the strength and toughness of the CPC. Creating submicron fibrous features in CPC was a useful method for enhancing the osteogenic differentiation and mineralization of stem cells. The novel electrospun fiber-CPC-hUCMSC construct is promising for stem cell delivery and bone tissue engineering.
机译：纤维支架由于其高表面积和模仿体内细胞外基质的纤维特征而有望用于组织工程。可以注射磷酸钙胶结剂（CPC）并在骨缺损中自我凝固。文献检索显示，尚无关于含电纺亚微米纤维的CPC上干细胞接种的报道。这项研究的目的是第一次研究CPC中的电纺纤维对力学性能和人脐带间充质干细胞（hUCMSC）增殖，成骨分化和矿化的影响。经由电纺丝技术制备聚（D，L-丙交酯-乙交酯）纤维，以产生650nm的平均纤维直径。将纤维掺入由磷酸四钙，无水磷酸二钙和壳聚糖乳酸组成的CPC中。纤维体积分数为0％，2.5％，5％和10％。含10％纤维的CPC的抗弯强度是不含纤维的CPC的两倍，其断裂强度（韧性）比不含纤维的CPC大一个数量级。当hUCMSCs附着到电纺纤维-CPC支架上时，迅速增殖并合成了骨矿物质。当CPC中的纤维体积分数从0％增加到10％时，hUCMSC的碱性磷酸酶，骨钙素和I型胶原的表达增加了一倍，而矿化增加40％。细胞功能增强归因于纤维CPC支架的高表面积和仿生特征。总之，将亚微米纤维掺入CPC可以大大提高CPC的强度和韧性。在CPC中创建亚微米纤维特征是增强干细胞成骨分化和矿化的有用方法。新型电纺纤维-CPC-hUCMSC构建体有望用于干细胞递送和骨组织工程。

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