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首页> 外文期刊>Acta biomaterialia >Electrospun nanofibrous scaffolds for controlled release of adeno-associated viral vectors.
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Electrospun nanofibrous scaffolds for controlled release of adeno-associated viral vectors.

机译：电纺纳米纤维支架，用于控制腺相关病毒载体的释放。

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The integration of viral gene delivery with key features of biomaterial scaffolds that modulate viral delivery in a controlled manner offers a promising strategy for numerous tissue engineering applications. In this study adeno-associated virus (AAV), which is widely utilized in human gene therapy as a gene carrier due to its safety and efficient gene delivery capability, was encapsulated within electrospun nanofibrous scaffolds composed of blended mixtures of elastin-like polypeptides (ELP) and poly (epsilon-caprolactone) (PCL) and was employed to transduce fibroblasts adherent on the scaffolds. Combinatorial interactions between ELP and PCL chains upon physical blending significantly altered the mechanical properties (i.e. wettability, elastic modulus, strain, etc.) of the ELP/PCL composites, thus providing key tools to mediate controlled release of AAV vectors and robust cellular transduction on the fibrous scaffolds. The ability of ELP/PCL composites to manipulate the controlled release of AAV-mediated gene delivery for subsequent high-efficiency cellular transduction will provide tremendous opportunities for a variety of tissue engineering applications.
机译：病毒基因传递与以受控方式调节病毒传递的生物材料支架的关键特征的集成为众多组织工程应用提供了一种有前途的策略。在这项研究中，腺伴随病毒（AAV）由于其安全性和高效的基因传递能力而被广泛用作人类基因治疗的基因载体，被封装在由弹性蛋白样多肽（ELP）混合混合物组成的电纺纳米纤维支架中）和聚（ε-己内酯）（PCL），并用于转导粘附在支架上的成纤维细胞。物理掺混后，ELP和PCL链之间的组合相互作用显着改变了ELP / PCL复合材料的机械性能（即，润湿性，弹性模量，应变等），从而提供了关键工具来介导AAV载体的受控释放和对AAV载体的稳健细胞转导。纤维支架。 ELP / PCL复合材料操纵AAV介导的基因释放受控释放以进行后续高效细胞转导的能力将为各种组织工程应用提供巨大的机会。

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