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首页> 外文期刊>Acta biomaterialia >Cell patterning using molecular vapor deposition of self-assembled monolayers and lift-off technique.
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Cell patterning using molecular vapor deposition of self-assembled monolayers and lift-off technique.

机译：使用分子分子气相沉积自组装单层和剥离技术进行细胞图案化。

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摘要

This paper reports a precise, live cell-patterning method by means of patterning a silicon or glass substrate with alternating cytophilic and cytophobic self-assembled monolayers (SAMs) deposited via molecular vapor deposition. Specifically, a stack of hydrophobic heptadecafluoro-1,1,2,2-tetrahydrodecyltrichlorosilane SAMs and a silicon oxide adhesion layer were patterned on the substrate surface, and a hydrophilic SAM derived from 3-trimethoxysilyl propyldiethylenetriamine was coated on the remaining non-treated areas on the substrate surface to promote cell growth. The primary characteristics of the reported method include: (i) single-cell resolution; (ii) easy alignment of the patterns with the pre-existing patterns on the substrate; (iii) easy formation of nanoscale patterns (depending on the exposure equipment); (iv) long shelf life of the substrate pattern prior to cell culturing; (v) compatibility with conventional, inverted, optical microscopes for simple visualization of patterns formed on a glass wafer; and (vi) the ability to support patterned cell (osteoblast) networks for at least 2 weeks. Here, we describe the deposition technique and the characterization of the deposited layers, as well as the application of this method in the fabrication of multielectrode arrays supporting patterned neuronal networks.
机译：本文报道了一种精确的活细胞构图方法，该方法是通过在硅或玻璃衬底上构图，并通过分子气相沉积沉积交替的亲细胞和疏亲自组装单分子层（SAMs）来进行构图。具体地，将疏水性七氟-1,1,2,2-四氢癸基三氯硅烷SAMs和氧化硅粘附层的堆叠图案化在基底表面上，并且将源自3-三甲氧基甲硅烷基丙基二亚乙基三胺的亲水性SAM涂覆在剩余的未处理区域上。在底物表面上促进细胞生长。报告的方法的主要特征包括：（i）单细胞分辨率; （ii）容易将图案与基板上预先存在的图案对准; （iii）容易形成纳米级图案（取决于曝光设备）; （iv）细胞培养之前底物图案的保质期长; （v）与传统的倒置光学显微镜兼容，以简单地观察玻璃晶片上形成的图案; （vi）支持图案化细胞（成骨细胞）网络至少2周的能力。在这里，我们描述了沉积技术和沉积层的表征，以及该方法在支持带图案的神经元网络的多电极阵列的制造中的应用。

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