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首页> 外文期刊>Acta biomaterialia >Topography and biocompatibility of patterned hydrophobic/hydrophilic zein layers.
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Topography and biocompatibility of patterned hydrophobic/hydrophilic zein layers.

机译：图案化的疏水/亲水玉米醇溶蛋白层的形貌和生物相容性。

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The topography and biocompatibility of zein layers adsorbed on patterned templates containing hydrophilic and hydrophobic regions were investigated. Nanopatterned templates consisting of hydrophilic lines on a hydrophobic background were drawn by dip-pen nanolithography (DPN) on gold-coated surfaces. 16-Mercaptohexadecanoic acid (COOH(CH(2))(15)SH, MHA) was used as primary ink to generate hydrophilic lines. Unpatterned surfaces were backfilled with 18-octadecanethiol (CH(3)(CH(2))(17)SH, ODT), which generated hydrophobic regions. Zein was allowed to adsorb on patterned surfaces from alcohol-water solutions. The topography of zein deposits was observed by atomic force microscopy (AFM). Height profiles from AFM measurements revealed that zein deposits followed closely the nanopatterned templates. The biocompatibility of zein layers assembled over hydrophilic/hydrophobic micropatterned templates was investigated. Templates containing MHA lines and ODT regions were generated by micro-contact printing (muCP). Mouse fibroblasts seeded on patterned zein layers proliferated on zein deposited over MHA lines, but not on zein over ODT. The experiment indicated that fibroblast cells were able to respond to variations in the underlying surface chemistry, transmitted by the different orientation adopted by zein on the different substrates. This property may be useful in controlling the spatial distribution of cells on patterned protein layers.
机译：研究了玉米蛋白层吸附在包含亲水和疏水区域的图案化模板上的形貌和生物相容性。通过浸涂式纳米光刻（DPN）在镀金表面上绘制由疏水背景上的亲水线组成的纳米图案模板。 16-巯基十六烷酸（COOH（CH（2））（15）SH，MHA）被用作主要墨水以产生亲水性线条。未图案化的表面回填18-十八烷硫醇（CH（3）（CH（2））（17）SH，ODT），生成疏水区域。玉米醇溶蛋白从醇-水溶液吸附在图案化的表面上。玉米醇溶蛋白沉积物的形貌通过原子力显微镜（AFM）观察。原子力显微镜测量的高度剖面表明，玉米醇溶蛋白沉积物紧随纳米图案模板。研究了在亲水/疏水微图案化模板上组装的玉米醇溶蛋白层的生物相容性。通过微接触印刷（muCP）生成包含MHA线和ODT区域的模板。接种在图案化玉米醇溶蛋白层上的小鼠成纤维细胞在沉积在MHA品系上的玉米醇溶蛋白上增殖，而在ODT上的玉米醇溶蛋白上不增殖。实验表明，成纤维细胞能够响应玉米醇溶蛋白在不同底物上采用的不同方向传递的潜在表面化学变化。此属性可能对控制图案化蛋白质层上细胞的空间分布有用。

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