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首页> 外文期刊>Acta biomaterialia >Biomimetic modification of the TiO(2)/glass composite Ecopore with heparinized collagen and the osteoinductive factor BMP-2.
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Biomimetic modification of the TiO(2)/glass composite Ecopore with heparinized collagen and the osteoinductive factor BMP-2.

机译：仿生修饰的TiO（2）/玻璃复合Ecopore与肝素化的胶原蛋白和骨诱导因子BMP-2。

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摘要

The porous TiO(2)/glass composite Ecopore has potential applications in hard tissue replacement. We describe the modification of Ecopore with the growth factor bone morphogenetic protein-2 (BMP-2) to add osteoinductive properties. Ecopore covalently coated with BMP-2 caused a weak induction of alkaline phosphatase in murine embryonal fibroblasts. In a rabbit bone defect model, BMP-2-coated Ecopore had moderately higher bone apposition rates and ingrown bone quantities at 6 weeks after implantation. To overcome loss of function due to chemical surface coupling, we filled the pore system of Ecopore with heparinized collagen sponge and loaded this secondary matrix with BMP-2. Heparinization of collagen filling increased the BMP-2 loading capacity of the matrix approximately 1.28-fold. Within 96h, 17.0+/-0.1 and 10.1+/-0.2% of the used BMP-2 was released from non-modified and heparinized Ecopore/collagen, respectively, indicating that the heparin modification retarded BMP-2 release. Revealed by energy-dispersive X-ray spectroscopy analysis of implant cross-sectional areas, BMP-2-loaded Ecopore/collagen had significantly higher bony ingrowth quantities in rabbits, with the heparinized modification yielding the highest value (16.09+/-3.51%, p<0.005) compared with the non-heparinized matrix (10.72+/-4.07%, p<0.05) and the BMP-2-free controls (5.60+/-1.47%). This suggested a beneficial effect of the biomimetic modification of Ecopore with heparinized collagen for bone healing and integration.
机译：多孔TiO（2）/玻璃复合材料Ecopore在硬组织替代中具有潜在的应用。我们描述了用生长因子骨形态发生蛋白2（BMP-2）对Ecopore的修饰，以增加骨诱导特性。用BMP-2共价包被的Ecopore在鼠胚胎成纤维细胞中引起碱性磷酸酶的弱诱导。在兔骨缺损模型中，植入BMP-2的Ecopore在植入后6周时具有适度较高的骨附着率和骨长大。为了克服由于化学表面偶联导致的功能丧失，我们用肝素化的胶原蛋白海绵填充了Ecopore的孔系统，并在此二级基质中加载了BMP-2。胶原蛋白填充的肝素化使基质的BMP-2负载量增加了约1.28倍。在96小时内，分别从未修饰的和肝素化的Ecopore /胶原释放了17.0 +/- 0.1％和10.1 +/- 0.2％的使用过的BMP-2，表明肝素修饰延迟了BMP-2的释放。通过对植入物横截面积的能量色散X射线光谱分析发现，负载BMP-2的Ecopore /胶原蛋白在兔中的骨向内生长量明显更高，其中肝素化修饰物产生的最高值（16.09 +/- 3.51％，与未肝素化的基质（10.72 +/- 4.07％，p <0.05）和不含BMP-2的对照（5.60 +/- 1.47％）相比，p <0.005）。这表明用肝素化的胶原蛋白对Ecopore进行仿生修饰对骨骼愈合和整合具有有益的作用。

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