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首页> 外文期刊>Acta biomaterialia >Dynamic culture enhances stem cell infiltration and modulates extracellular matrix production on aligned electrospun nanofibrous scaffolds.
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Dynamic culture enhances stem cell infiltration and modulates extracellular matrix production on aligned electrospun nanofibrous scaffolds.

机译：动态培养可增强干细胞浸润并调节对齐的电纺纳米纤维支架上的细胞外基质产生。

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Electrospun nanofibrous scaffolds have become widely investigated for tissue engineering applications, owing to their ability to replicate the scale and organization of many fiber-reinforced soft tissues such as the knee meniscus, the annulus fibrosus of the intervertebral disc, tendon, and cartilage. However, due to their small pore size and dense packing of fibers, cellular ingress into electrospun scaffolds is limited. Progress in the application of electrospun scaffolds has therefore been hampered, as limited cell infiltration results in heterogeneous deposition of extracellular matrix and mechanical properties that remain below native benchmarks. In the present study, dynamic culture conditions dramatically improved the infiltration of mesenchymal stem cells into aligned nanofibrous scaffolds. While dynamic culture resulted in a reduction of glycosaminoglycan content, removal from dynamic culture to free-swelling conditions after 6 weeks resulted recovery of glycosaminoglycan content. Dynamic culture significantly increased collagen content, and collagen was more uniformly distributed throughout the scaffold thickness. While mechanical function was assessed and tensile modulus increased with culture duration, dynamic culture did not result in any additional improvement beyond free-swelling culture. Transient dynamic (6 weeks dynamic followed by 6 weeks free-swelling) culture significantly enhanced cell infiltration while permitting GAG accumulation. In this study, we demonstrated that a simple modification to standard in vitro culture conditions effectively improves cellular ingress into electrospun scaffolds, resolving a challenge which has until now limited the utility of these materials for various tissue engineering applications.
机译：静电纺丝纳米纤维支架由于能够复制许多纤维增强的软组织（如膝半月板，椎间盘环纤维，腱和软骨）的规模和组织能力，因此已被广泛用于组织工程研究。然而，由于它们的小孔径和密集的纤维堆积，细胞进入电纺支架的进入受到限制。由于有限的细胞浸润导致细胞外基质的异质沉积和机械性能仍低于天然基准，因此电纺支架的应用进展受到了阻碍。在本研究中，动态培养条件显着改善了间充质干细胞向对齐的纳米纤维支架中的浸润。虽然动态培养导致糖胺聚糖含量降低，但是在6周后从动态培养移至自由溶胀条件下，糖胺聚糖含量得以恢复。动态培养显着增加了胶原蛋白含量，并且胶原蛋白在整个支架厚度中更均匀地分布。虽然评估了机械功能，并且拉伸模量随培养时间的延长而增加，但动态培养并未导致自由溶胀培养以外的任何其他改善。瞬时动态（动态6周，然后自由膨胀6周）培养可显着增强细胞浸润，同时允许GAG积累。在这项研究中，我们证明了对标准体外培养条件的简单修改有效地改善了细胞进入电纺支架的过程，解决了迄今为止一直限制了这些材料在各种组织工程应用中的实用性的挑战。

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