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首页> 外文期刊>Acta biomaterialia >Regeneration of the femoral epicondyle on calcium-binding silk scaffolds developed using transgenic silk fibroin produced by transgenic silkworm.
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Regeneration of the femoral epicondyle on calcium-binding silk scaffolds developed using transgenic silk fibroin produced by transgenic silkworm.

机译：使用转基因蚕产生的转基因丝素蛋白开发的钙结合丝支架上股上epi的再生。

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摘要

Genetically modified silk fibroin containing a poly-glutamic acid site, [(AGSGAG)4E8AS]4, for mineralization was produced as fibers by transgenic silkworms through systematic transformation of the silkworms. The Ca binding activity and mineralization of the transgenic silk fibroin were examined in vitro, showing that this transgenic silk fibroin had relatively high Ca binding activity compared with native silk fibroin. Porous silk scaffolds were prepared with the transgenic and native silk fibroins. Healing of femoral epicondyle defects in rabbit femurs treated with the scaffolds was examined by observing changes in images of the defects using micro-computed tomography. Earlier mineralization and bone formation were observed with scaffolds of transgenic silk fibroin compared with those of native silk fibroin. Thus, this study shows the feasibility of using genetically modified silk fibroin from transgenic silkworms as a mineralization-accelerating material for bone repair.
机译：转基因蚕通过对蚕进行系统转化，将含有聚谷氨酸位点[（AGSGAGAG）4E8AS] 4的转基因蚕丝蛋白制成纤维。在体外检查了转基因丝素蛋白的Ca结合活性和矿化作用，表明该转基因丝素蛋白与天然丝素蛋白相比具有较高的Ca结合活性。用转基因的和天然的丝素蛋白制备多孔的丝支架。通过使用微型计算机断层摄影术观察缺陷图像的变化，检查用支架治疗的兔股骨的股骨上con缺损的愈合情况。与天然丝素蛋白的支架相比，转基因丝素蛋白的支架观察到更早的矿化和骨形成。因此，这项研究表明使用转基因蚕的转基因丝素蛋白作为矿化促进材料进行骨修复的可行性。

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