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首页> 外文期刊>Acta biomaterialia >Development and optimization of a dual-photoinitiator, emulsion-based technique for rapid generation of cell-laden hydrogel microspheres.
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Development and optimization of a dual-photoinitiator, emulsion-based technique for rapid generation of cell-laden hydrogel microspheres.

机译：开发和优化基于光引发剂的双光引发剂，可快速生成充满细胞的水凝胶微球。

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A growing number of clinical trials explore the use of cell-based therapies for the treatment of disease and restoration of damaged tissue; however, limited cell survival and engraftment remains a significant challenge. As the field continues to progress, microencapsulation strategies are proving to be a valuable tool for protecting and supporting these cell therapies while preserving minimally invasive delivery. This work presents a novel, dual-photoinitiator technique for encapsulation of cells within hydrogel microspheres. A desktop vortexer was used to generate an emulsion of poly(ethylene glycol) diacrylate (PEGDA) or PEGDA-based precursor solution in mineral oil. Through an optimized combination of photoinitiators added to both the aqueous and the oil phase, rapid gelation of the suspended polymer droplets was achieved. The photoinitiator combination provided superior cross-linking consistency and greater particle yield, and required lower overall initiator concentrations compared with a single initiator system. When cells were combined with the precursor solution, these benefits translated to excellent microencapsulation yield with 60-80% viability for the tested cell types. It was further shown that the scaffold material could be modified with cell-adhesive peptides to be used as surface-seeded microcarriers, or additionally with enzymatically degradable sequences to support three-dimensional spreading, migration and long-term culture of encapsulated cells. Three cell lines relevant to neural stem cell therapies are demonstrated here, but this technology is adaptable, scalable and easy to implement with standard laboratory equipment, making it a useful tool for advancing the next generation of cell-based therapeutics.
机译：越来越多的临床试验探索使用基于细胞的疗法来治疗疾病和受损组织的恢复。然而，有限的细胞存活和植入仍然是一个重大挑战。随着该领域的不断发展，微囊化策略已被证明是保护和支持这些细胞疗法同时保持微创递送的有价值的工具。这项工作提出了一种新颖的双重光引发剂技术，用于将细胞封装在水凝胶微球内。使用台式涡旋仪产生聚（乙二醇）二丙烯酸酯（PEGDA）或基于PEGDA的前体溶液在矿物油中的乳液。通过添加到水相和油相中的光引发剂的优化组合，可以实现悬浮的聚合物液滴的快速胶凝。与单一引发剂体系相比，光引发剂组合可提供优异的交联稠度和更高的颗粒收率，并需要较低的总引发剂浓度。当将细胞与前体溶液合并后，这些好处转化为出色的微囊化产率，对于测试的细胞类型，其活力为60-80％。进一步显示，可以用细胞粘附肽修饰支架材料以用作表面接种的微载体，或者另外可以用酶促降解序列进行修饰，以支持封装细胞的三维扩散，迁移和长期培养。这里展示了与神经干细胞疗法相关的三种细胞系，但是该技术具有适应性，可扩展性，并且易于通过标准实验室设备实施，使其成为推进下一代基于细胞的疗法的有用工具。

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