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首页> 外文期刊>Acta biomaterialia >Three-dimensional biodegradable microscaffolding: scaffold characterization and cell population at single cell resolution.
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Three-dimensional biodegradable microscaffolding: scaffold characterization and cell population at single cell resolution.

机译：三维可生物降解的微支架：支架特性和单细胞分辨率下的细胞群体。

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摘要

Engineering artificial tissue scaffolds with a similar organization to that of the natural tissue is a key element to the successful recapitulation of function. However, three-dimensional (3-D) fabrication of tissue scaffolds containing complex microarchitectures still remains a challenge. In addition, little attention has been paid to the issue of how to incorporate cells within 3-D tissue scaffolds that contain precisely engineered architectures. Here we report a 3-D biodegradable microscaffolding (3D-BMS) technology and its process characterization as well as a microscale cellular loading technology as an efficient way to massively populate biodegradable polymers with cells at single cell resolution. In this study a particular emphasis was given to characterization of the material properties of the biodegradable polymers undergoing the 3D-BMS processes. Optimal process conditions were identified in order to avoid any unwanted change in material properties, such as crystallinity and scaffold strength, that have a direct impact on the degradation speed and physical integrity of the constructed scaffolds. For precise control of the cell distribution within the microstructured scaffolds a high precision microsieve structure was designed to localize rat hepatocytes and human articular chondrocytes in the biodegradable polymers. Cell suspensions were passed at a predetermined flow rate through biodegradable polymer layers that contained tapered microholes in a massively parallel process. This high resolution cell seeding method allows accurate manipulation of cell placement in thin layers of biodegradable polymers.
机译：工程化的人造组织支架具有与天然组织相似的组织，是成功再现功能的关键要素。然而，包含复杂的微结构的组织支架的三维（3-D）制造仍然是一个挑战。此外，很少关注如何将细胞整合到包含精确工程架构的3D组织支架中。在这里，我们报告3-D生物可降解微支架（3D-BMS）技术及其过程表征以及微型细胞加载技术，作为在单细胞分辨率下大量填充可生物降解聚合物的有效方法。在这项研究中，特别强调了经历3D-BMS过程的可生物降解聚合物的材料特性的表征。确定最佳工艺条件，以避免材料性能的任何不想要的变化，例如结晶度和支架强度，这些变化直接影响所构建支架的降解速度和物理完整性。为了精确控制微结构支架内的细胞分布，设计了高精度微筛结构以将大鼠肝细胞和人关节软骨细胞定位在可生物降解的聚合物中。使细胞悬液以预定的流速通过大规模平行过程通过包含锥形微孔的可生物降解聚合物层。这种高分辨率的细胞接种方法可以精确控制可生物降解聚合物薄层中细胞的放置。

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