• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Fluorescence labeling of colloidal core-shell particles with defined isoelectric points for in vitro studies
【24h】

Fluorescence labeling of colloidal core-shell particles with defined isoelectric points for in vitro studies

机译：具有确定的等电点的胶体核-壳颗粒的荧光标记，用于体外研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In the light of in vitro nanotoxicological studies fluorescence labeling has become standard for particle localization within the cell environment. However, fluorescent labeling is also known to significantly alter the particle surface chemistry and therefore potentially affect the outcome of cell studies. Hence, fluorescent labeling is ideally carried out without changing, for example, the isoelectric point. A simple and straightforward method for obtaining fluorescently labeled spherical metal oxide particles with well-defined isoelectric points and a narrow size distribution is presented in this study. Spherical amorphous silica (SiO 2, 161 nm diameter) particles were used as the substrate material and were coated with silica, alumina (Al 2O 3), titania (TiO 2), or zirconia (ZrO 2) using sol-gel chemistry. Fluorescent labeling was achieved by directly embedding rhodamine 6G dye in the coating matrix without affecting the isoelectric point of the metal oxide coatings. The coating quality was confirmed by high resolution transmission electron microscopy, energy filtered transmission electron microscopy and electrochemical characterization. The coatings were proven to be stable for at least 240 h under different pH conditions. The well-defined fluorescent particles can be directly used for biomedical investigations, e.g. elucidation of particle-cell interactions in vitro.
机译：根据体外纳米毒理学研究，荧光标记已成为细胞环境中颗粒定位的标准。但是，也已知荧光标记会显着改变颗粒表面化学性质，因此可能会影响细胞研究的结果。因此，理想地进行荧光标记而不改变例如等电点。在这项研究中提出了一种简单直接的方法来获得荧光标记的球形金属氧化物粒子，该粒子具有明确的等电点和狭窄的尺寸分布。使用球形无定形二氧化硅（SiO 2，直径161 nm）作为基质材料，并使用溶胶-凝胶化学方法将二氧化硅，氧化铝（Al 2O 3），二氧化钛（TiO 2）或氧化锆（ZrO 2）涂覆。荧光标记是通过将罗丹明6G染料直接嵌入涂层基质中而不会影响金属氧化物涂层的等电点而实现的。通过高分辨率透射电子显微镜，能量过滤的透射电子显微镜和电化学表征来确认涂层质量。事实证明，该涂料在不同的pH条件下可稳定至少240小时。定义明确的荧光颗粒可直接用于生物医学研究，例如阐明体外的颗粒-细胞相互作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号