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首页> 外文期刊>Acta biomaterialia >Visible light crosslinkable chitosan hydrogels for tissue engineering
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Visible light crosslinkable chitosan hydrogels for tissue engineering

机译：用于组织工程的可见光可交联壳聚糖水凝胶

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In situ gelling constructs, which form a hydrogel at the site of injection, offer the advantage of delivering cells and growth factors to the complex structure of the defect area for tissue engineering. In the present study, visible light crosslinkable hydrogel systems were presented using methacrylated glycol chitosan (MeGC) and three blue light initiators: camphorquinone (CQ), fluorescein (FR) and riboflavin (RF). A minimal irradiation time of 120 s was required to produce MeGC gels able to encapsulate cells with CQ or FR. Although prolonged irradiation up to 600 s improved the mechanical strength of CQ- or FR-initiated gels (compressive modulus 2.8 or 4.4 kPa, respectively), these conditions drastically reduced encapsulated chondrocyte viability to 5% and 25% for CQ and FR, respectively. Stable gels with 80-90% cell viability could be constructed using radiofrequency (RF) with only 40 s irradiation time. Increasing irradiation time up to 300 s significantly improved the compressive modulus of the RF-initiated MeGC gels up to 8.5 kPa without reducing cell viability. The swelling ratio and degradation rate were smaller at higher irradiation time. RF-photoinitiated hydrogels supported proliferation of encapsulated chondrocytes and extracellular matrix deposition. The feasibility of this photoinitiating system as in situ gelling hydrogels was further demonstrated in osteochondral and chondral defect models for potential cartilage tissue engineering. The MeGC hydrogels using RF offer a promising photoinitiating system in tissue engineering applications.
机译：原位胶凝构建体在注射部位形成水凝胶，具有将细胞和生长因子输送到缺损区域的复杂结构以进行组织工程的优势。在本研究中，提出了使用甲基丙烯酸二醇壳聚糖（MeGC）和三种蓝光引发剂：樟脑醌（CQ），荧光素（FR）和核黄素（RF）的可见光可交联水凝胶体系。制备能够用CQ或FR包裹细胞的MeGC凝胶最少需要120 s的照射时间。尽管延长辐照至600 s可以改善CQ或FR引发的凝胶的机械强度（压缩模量分别为2.8或4.4 kPa），但这些条件将CQ和FR的封装软骨细胞活力急剧降低至5％和25％。可以使用仅40 s照射时间的射频（RF）来构建具有80-90％细胞活力的稳定凝胶。延长照射时间至300 s可以显着提高RF引发的MeGC凝胶的压缩模量，最高至8.5 kPa，而不会降低细胞活力。较高的照射时间溶胀率和降解率较小。射频光引发的水凝胶支持封装的软骨细胞的增殖和细胞外基质的沉积。这种光引发体系作为原位胶凝水凝胶的可行性已在骨软骨和软骨缺损模型中用于潜在的软骨组织工程学进一步证明。使用RF的MeGC水凝胶在组织工程应用中提供了有希望的光引发系统。

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