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首页> 外文期刊>Acta biomaterialia >Engineered silica nanocarriers as a high-payload delivery vehicle for antioxidant enzymes
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Engineered silica nanocarriers as a high-payload delivery vehicle for antioxidant enzymes

机译：工程二氧化硅纳米载体可作为抗氧化酶的高有效载荷载体

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摘要

Antioxidant enzymes for the treatment of oxidative stress-related diseases remain a highly promising therapeutic approach. As poor localization and stability have been the greatest challenges to their clinical translation, a variety of nanocarrier systems have been developed to directly address these limitations. In most cases, there has been a trade-off between the delivered mass of enzyme loaded and the carriers ability to protect the enzyme from proteolytic degradation. One potential method of overcoming this limitation is the use of ordered mesoporous silica materials as potential antioxidant enzyme nanocarriers. The present study compared the loading, activity and retention activity of an anti-oxidant enzyme, catalase, on four engineered mesoporous silica types: non-porous silica particles, spherical silica particles with radially oriented pores and hollow spherical silica particles with pores oriented either parallel to the hollow core or expanded, interconnected bimodal pores. All these silica types, except non-porous silica, displayed potential for effective catalase loading and protection against the proteolytic enzyme, pronase. Hollow particles with interconnected pores exhibit protein loading of up to 50 wt.% carrier mass, while still maintaining significant protection against proteolysis.
机译：用于治疗与氧化应激相关的疾病的抗氧化酶仍然是非常有前途的治疗方法。由于不良的定位和稳定性是对其临床翻译的最大挑战，因此已经开发了各种纳米载体系统来直接解决这些局限性。在大多数情况下，在负载的酶的输送质量与载体之间的权衡取舍，载体保护酶免受蛋白水解降解的能力。克服该限制的一种潜在方法是使用有序介孔二氧化硅材料作为潜在的抗氧化酶纳米载体。本研究比较了抗氧化酶过氧化氢酶在四种工程介孔二氧化硅类型上的负载，活性和保留活性：无孔二氧化硅颗粒，具有径向取向孔的球形二氧化硅颗粒和具有平行取向的孔的中空球形二氧化硅颗粒到空心或膨胀的，相互连接的双峰孔隙。除无孔二氧化硅外，所有这些二氧化硅类型均显示出有效负载过氧化氢酶和保护蛋白水解酶，链霉蛋白酶的潜力。具有相互连接的孔的中空颗粒显示出高达50 wt。％的载体质量的蛋白质负载，同时仍保持了对蛋白水解的显着保护作用。

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