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首页> 外文期刊>Acta biomaterialia >Crosslinking strategies facilitate tunable structural properties of fibrin microthreads.
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Crosslinking strategies facilitate tunable structural properties of fibrin microthreads.

机译：交联策略促进了血纤蛋白微线的可调结构特性。

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摘要

A significant challenge in the design of biomimetic scaffolds is combining morphologic, mechanical, and biochemical cues into a single construct to promote tissue regeneration. In this study, we analyzed the effects of different crosslinking conditions on fibrin biopolymer microthreads to create morphologic scaffolds with tunable mechanical properties that are designed for directional cell guidance. Fibrin microthreads were crosslinked using carbodiimides in either acidic or neutral buffer, and the mechanical, structural, and biochemical responses of the microthreads were investigated. Crosslinking in the presence of acidic buffer (EDCa) created microthreads that had significantly higher tensile strengths and moduli than all other microthreads, and failed at lower strains than all other microthreads. Microthreads crosslinked in neutral buffer (EDCn) were also significantly stronger and stiffer than uncrosslinked threads and were comparable to contracting muscle in stiffness. Swelling ratios of crosslinked microthreads were significantly different from each other and uncrosslinked controls, suggesting a difference in the internal organization and compaction of the microthreads. Using an in vitro degradation assay, we observed that EDCn microthreads degraded within 24h, six times slower than uncrosslinked control threads, but EDCa microthreads did not show any significant indication of degradation within the 7-day assay period. Microthreads with higher stiffnesses supported significantly increased attachment of C2C12 cells, as well as increases in cell proliferation without a decrease in cell viability. Taken together, these data demonstrate the ability to create microthreads with tunable mechanical and structural properties that differentially direct cellular functions. Ultimately, we anticipate that we can strategically exploit these properties to promote site-specific tissue regeneration.
机译：仿生支架的设计中的一项重大挑战是将形态学，机械学和生化学方面的线索结合到单个构建物中以促进组织再生。在这项研究中，我们分析了不同的交联条件对血纤蛋白生物聚合物微丝的影响，以创建具有可调机械性能的形态学支架，这些支架被设计用于定向细胞指导。使用碳二亚胺在酸性或中性缓冲液中使纤维蛋白微丝交联，并研究了微丝的机械，结构和生化反应。在酸性缓冲液（EDCa）的存在下交联产生的微丝比所有其他微丝具有显着更高的拉伸强度和模量，并且在比所有其他微丝更低的应变下失效。在中性缓冲液（EDCn）中交联的微线也比未交联的线显着更强和更硬，并且与收缩肌肉相当。交联的微丝的溶胀率彼此之间和未交联的对照之间存在显着差异，表明微丝的内部组织和紧密度不同。使用体外降解分析，我们观察到EDCn微丝在24小时内降解，比未交联的对照丝慢六倍，但是EDCa微丝在7天的检测期内未显示任何明显的降解迹象。具有较高刚度的微线支持C2C12细胞的附着显着增加，以及细胞增殖的增加而细胞活力没有下降。综上所述，这些数据证明了创建具有可调节的机械和结构特性的微螺纹的能力，这些微螺纹可差异地指导细胞功能。最终，我们希望可以从战略上利用这些特性来促进特定部位的组织再生。

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