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首页> 外文期刊>Acta biomaterialia >Non-cross-linked porcine-based collagen I-III membranes do not require high vascularization rates for their integration within the implantation bed: A paradigm shift
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Non-cross-linked porcine-based collagen I-III membranes do not require high vascularization rates for their integration within the implantation bed: A paradigm shift

机译：非交联的基于猪的胶原蛋白I-III膜不需要很高的血管形成速率即可整合到植入床中：范例转变

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There are conflicting reports concerning the tissue reaction of small animals to porcine-based, non-cross-linked collagen I-III membranes/matrices for use in guided tissue/bone regeneration. The fast degradation of these membranes/matrices combined with transmembrane vascularization within 4 weeks has been observed in rats compared with the slow vascularization and continuous integration observed in mice. The aim of the present study was to analyze the tissue reaction to a porcine-based non-cross-linked collagen I-III membrane in mice. Using a subcutaneous implantation model, the membrane was implanted subcutaneously in mice for up to 60 days. The extent of scaffold vascularization, tissue integration and scaffold thickness were assessed using general and specialized histological methods, together with a unique histomorphometrical analysis technique. A dense Bombyx mori-derived silk fibroin membrane was used as a positive control, whilst a polytetrafluoroethylene (PTFE) membrane served as a negative control. Within the observation period, the collagen membrane induced a mononuclear cellular tissue response, including anti-inflammatory macrophages and the absence of multinucleated giant cells within its implantation bed. Transmembrane scaffold vascularization was not observed, whereas a mild scaffold vascularization was generated through microvessels located at both scaffold surfaces. However, the silk fibroin induced a mononuclear and multinucleated cell-based tissue response, in which pro-inflammatory macrophages and multinucleated giant cells were associated with an increasing transmembrane scaffold vascularization and a breakdown of the membrane within the experimental period. The PTFE membrane remained as a stable barrier throughout the study, and visible cellular degradation was not observed. However, multinucleated giant cells were located on both interfaces. The present study demonstrated that the tested non-cross-linked collagen membrane remained as a stable barrier membrane throughout the study period. The membrane integrated into the subcutaneous connective tissue and exhibited only a mild peripheral vascularization without experiencing breakdown. The silk fibroin, in contrast, induced granulation tissue formation, which resulted in its high vascularization and the breakdown of the material over time. The presence of multinucleated giant cells at both interfaces of the PFTE membrane is a sign of its slow cellular biodegradation and might lead to adhesions between the membrane and its surrounding tissue. This hypothesis could explain the observed clinical complications associated with the retrieval of these materials after guided tissue regeneration.
机译：关于小动物对基于猪的非交联胶原I-III膜/基质的组织反应的矛盾报道，用于引导组织/骨再生。与在小鼠中观察到的缓慢血管化和持续整合相比，在大鼠中观察到这些膜/基质在4周内快速降解并与跨膜血管化相结合。本研究的目的是分析小鼠中基于猪的非交联胶原I-III膜的组织反应。使用皮下植入模型，将膜皮下植入小鼠体内长达60天。使用一般和专门的组织学方法，以及独特的组织形态分析技术，评估支架血管化程度，组织整合和支架厚度。致密的Bombyx桑蚕丝素蛋白膜用作阳性对照，而聚四氟乙烯（PTFE）膜用作阴性对照。在观察期内，胶原膜诱导了单核细胞组织反应，包括抗炎性巨噬细胞和植入床内不存在多核巨细胞。没有观察到跨膜支架血管化，而通过位于两个支架表面的微血管产生了轻度支架血管化。然而，丝素蛋白诱导基于单核和多核细胞的组织反应，其中促炎性巨噬细胞和多核巨细胞与跨膜支架血管化的增加和在实验期内膜的破裂有关。在整个研究过程中，PTFE膜仍是稳定的屏障，未观察到可见的细胞降解。但是，多核巨细胞位于两个接口上。本研究表明，在整个研究期间，被测试的非交联胶原蛋白膜仍作为稳定的屏障膜。该膜整合到皮下结缔组织中，仅表现出轻度的外周血管形成，而没有发生破裂。相反，丝素蛋白诱导肉芽组织形成，这导致其高血管化和材料随时间分解。 PFTE膜的两个界面处都存在多核巨细胞，这是其缓慢的细胞生物降解的迹象，并可能导致膜与周围组织之间的粘附。该假设可以解释在指导的组织再生后观察到的与这些材料的取出有关的临床并发症。

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