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首页> 外文期刊>Acta biomaterialia >Nanofiber size-dependent sensitivity of fibroblast directionality to the methodology for scaffold alignment.
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Nanofiber size-dependent sensitivity of fibroblast directionality to the methodology for scaffold alignment.

机译：纳米纤维大小依赖成纤维细胞方向性对支架对齐方法的敏感性。

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The sensitivity of fibroblast guidance on directional cues provided by aligned nanofibers is studied for scaffolds of successively smaller fiber sizes (740±280, 245±85, 140±40, and 80±10 nm) fabricated using mandrel and electrical alignment methodologies for electrospun nanofibers (～10° angular deviation (AD)), as well as nanoimprint methodologies for perfectly aligned fibers (0° AD). On aligned scaffolds of large fibers (～740 nm) cell directionality closely follows the underlying fibers, irrespective of the alignment method. However, on mandrel aligned scaffolds of successively smaller fibers the cell directionality exhibits greater deviations from the underlying fiber alignment due to the higher likelihood of interaction of cell lamellipodia with multiple, rather than single, nanofibers. Using electrically aligned scaffolds, fibroblast directionality deviations can be maintained in the range of nanofiber alignment deviation for fiber sizes down to ～100 nm. This improvement in cell guidance is attributed to molecular scale directional adhesion cues for cell receptors, which occur within electrically aligned scaffolds due to fiber polarization parallel to the geometric alignment axis of the nanofiber under the modified electric field during electrospinning. While fibroblast directionality is similar on electrically aligned vs. nanoimprinted scaffolds for fiber sizes >100 nm, cell directionality is influenced more strongly by the perfect alignment cues of the latter on ～100 nm fiber scaffolds. The scaffold alignment methodology is hence highly significant, especially for tissue engineering applications requiring sub-100 nm aligned fibers.
机译：研究了使用心轴和电对准方法为静电纺丝纳米纤维制造的依次较小纤维尺寸（740±280、245±85、140±40和80±10 nm）的依次较小的支架，研究了由对齐的纳米纤维提供的成纤维细胞引导对定向线索的敏感性。 （〜10°角度偏差（AD）），以及用于完美对齐的光纤（0°AD）的纳米压印方法。在对齐的大纤维支架（约740 nm）上，细胞的方向性紧密跟随下面的纤维，而与对齐方法无关。然而，在依次较小纤维的心轴对准的支架上，由于细胞板状脂膜细胞与多根而不是单根纳米纤维相互作用的可能性更高，因此细胞方向性表现出与下面的纤维排列更大的偏离。使用电对准的支架，对于直径低至约100 nm的纤维，成纤维细胞方向性偏差可保持在纳米纤维对准偏差的范围内。细胞引导的这种改善归因于细胞受体的分子尺度方向粘附线索，由于在电纺丝过程中在平行电场下平行于纳米纤维的几何排列轴的纤维极化，在电排列的支架内发生。当纤维尺寸> 100 nm时，电排列的支架与纳米压印支架上的成纤维细胞方向性相似，而细胞的方向性则受后者在〜100 nm纤维支架上的完美排列提示的影响更大。因此，支架对准方法非常重要，特别是对于需要低于100 nm对准光纤的组织工程应用而言。

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