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首页> 外文期刊>Acta biomaterialia >Umbilical cord stem cells released from alginate-fibrin microbeads inside macroporous and biofunctionalized calcium phosphate cement for bone regeneration
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Umbilical cord stem cells released from alginate-fibrin microbeads inside macroporous and biofunctionalized calcium phosphate cement for bone regeneration

机译：从大孔和生物功能化磷酸钙水泥中的藻酸盐-纤维蛋白微珠释放的脐带干细胞，用于骨骼再生

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摘要

The need for bone repair has increased as the population ages. The objectives of this study were to (1) develop a novel biofunctionalized and macroporous calcium phosphate cement (CPC) containing alginate-fibrin microbeads encapsulating human umbilical cord mesenchymal stem cells (hUCMSC) and, for the first time, (2) investigate hUCMSC proliferation and osteogenic differentiation inside the CPC. A macroporous CPC was developed using calcium phosphate powder, chitosan, and a gas-foaming porogen. Five types of CPC were fabricated: a CPC control, CPC + 0.05% fibronectin (Fn), CPC + 0.1% Fn, CPC + 0.1% arginine-glycine-aspartate (RGD), and CPC + 0.1% Fn + 0.1% RGD. Alginate-fibrin microbeads containing 10 6 hUCMSC per ml were encapsulated in the CPC paste. After the CPC had set, the degradable microbeads released hUCMSC within it. The hUCMSC proliferated inside the CPC, with the cell density after 21 days being 4-fold that on day1. CPC + 0.1% RGD had the highest cell density, which was 4-fold that of the CPC control. The released cells differentiated along the osteogenic lineage and synthesized bone mineral. The hUCMSC inside the CPC + 0.1% RGD construct expressed the genes alkaline phosphatase, osteocalcin and collagen I, at twice the level of the CPC control. Mineral synthesis by hUCMSC inside the CPC + 0.1% RGD construct was 2-fold that in the CPC control. RGD and Fn incorporation in the CPC did not compromise its strength, which matched the reported strength of cancellous bone. In conclusion, degradable microbeads released hUCMSC which proliferated, differentiated and synthesized minerals inside the macroporous CPC. The CPC with RGD greatly enhanced cell function. The novel biofunctionalized and macroporous CPC-microbead-hUCMSC construct is promising for bone tissue engineering applications.
机译：随着人口老龄化，对骨修复的需求增加了。这项研究的目的是（1）开发一种新型的生物功能化的大孔磷酸钙水泥（CPC），其中包含藻酸盐-血纤蛋白微珠，其包裹人脐带间充质干细胞（hUCMSC），并且（2）首次研究了hUCMSC增殖以及CPC内部的成骨分化。使用磷酸钙粉末，壳聚糖和泡沫发泡成孔剂开发了大孔CPC。制作了五种类型的CPC：CPC对照，CPC + 0.05％纤连蛋白（Fn），CPC + 0.1％Fn，CPC + 0.1％精氨酸-甘氨酸-天冬氨酸（RGD）和CPC + 0.1％Fn + 0.1％RGD。将每毫升含10 6 hUCMSC的藻酸盐-纤维蛋白微珠封装在CPC糊剂中。 CPC凝固后，可降解的微珠在其中释放了hUCMSC。 hUCMSC在CPC内增殖，21天后的细胞密度是第1天的4倍。 CPC + 0.1％RGD的细胞密度最高，是CPC对照的4倍。释放的细胞沿成骨细胞系分化并合成骨矿物质。 CPC + 0.1％RGD构建体中的hUCMSC表达了碱性磷酸酶，骨钙素和胶原I基因，是CPC对照水平的两倍。 CPC + 0.1％RGD构建体内部的hUCMSC矿物合成是CPC对照的2倍。 RGD和Fn掺入CPC中并没有影响其强度，这与报道的松质骨强度相符。总之，可降解的微珠释放了hUCMSC，后者在大孔CPC内部增殖，分化和合成了矿物质。带有RGD的CPC大大增强了细胞功能。新型的生物功能化和大孔CPC-microbead-hUCMSC构建体有望用于骨组织工程应用。

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