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首页> 外文期刊>Acta biomaterialia >A substrate-independent method for surface grafting polymer layers by atom transfer radical polymerization: Reduction of protein adsorption
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A substrate-independent method for surface grafting polymer layers by atom transfer radical polymerization: Reduction of protein adsorption

机译：通过原子转移自由基聚合表面接枝聚合物层的独立于基材的方法：减少蛋白质吸附

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摘要

A general method for producing low-fouling biomaterials on any surface by surface-initiated grafting of polymer brushes is presented. Our procedure uses radiofrequency glow discharge thin film deposition followed by macro-initiator coupling and then surface-initiated atom transfer radical polymerization (SI-ATRP) to prepare neutral polymer brushes on planar substrates. Coatings were produced on substrates with variable interfacial composition and mechanical properties such as hard inorganic/metal substrates (silicon and gold) or flexible (perfluorinated poly(ethylene-co-propylene) film) and rigid (microtitre plates) polymeric materials. First, surfaces were functionalized via deposition of an allylamine plasma polymer thin film followed by covalent coupling of a macro-initiator composed partly of ATRP initiator groups. Successful grafting of a hydrophilic polymer layer was achieved by SI-ATRP of N,N′- dimethylacrylamide in aqueous media at room temperature. We exemplified how this method could be used to create surface coatings with significantly reduced protein adsorption on different material substrates. Protein binding experiments using labelled human serum albumin on grafted materials resulted in quantitative evidence for low-fouling compared to control surfaces.
机译：提出了通过表面引发的聚合物刷接枝在任何表面上生产低污染生物材料的一般方法。我们的程序使用射频辉光放电薄膜沉积，然后进行宏观引发剂偶联，然后进行表面引发的原子转移自由基聚合（SI-ATRP），以在平面基板上制备中性聚合物刷。在具有可变界面组成和机械性能的基材上生产涂层，例如硬无机/金属基材（硅和金）或柔性（全氟化聚（乙烯-丙烯）薄膜）和刚性（微量滴定板）聚合物材料。首先，通过沉积烯丙胺等离子体聚合物薄膜使表面官能化，然后共价偶联部分由ATRP引发剂基团组成的大分子引发剂。在室温下，通过N-N'-二甲基丙烯酰胺的SI-ATRP在水性介质中成功接枝了亲水性聚合物层。我们举例说明了该方法如何用于创建表面涂层，从而显着减少蛋白质在不同材料基材上的吸附。使用标记的人血清白蛋白在嫁接材料上进行的蛋白结合实验可得到与对照表面相比低结垢的定量证据。

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