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首页> 外文期刊>Acta biomaterialia >In vitro characterization and osteoblast responses to nanostructured photocatalytic TiO 2 coated surfaces
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In vitro characterization and osteoblast responses to nanostructured photocatalytic TiO 2 coated surfaces

机译：纳米结构光催化TiO 2涂层表面的体外表征和成骨细胞反应

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摘要

The aims of the study were to characterize a nanostructured photoactive titanium dioxide (TiO 2) coating and to compare the cellular response of human osteoblasts before and after ultraviolet (UV) irradiation of the coating. A specific nanostructured TiO 2 powder (Degussa P-25), which consists of approximately 80% anatase and 20% rutile, was spin-coated onto commercially pure titanium discs, and was heat-treated thereafter. After topographical, chemical and photocatalytic property characterizations, human osteoblasts were cultured on the coated discs before and after UV irradiation. Cell morphology was evaluated by scanning electron microscopy (SEM), and cell viability was analysed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. From the contact angle analysis, the wettability significantly improved after UV irradiation. The cultured cells were flattened with numerous elongated lammellipodia; however, no morphological differences were indicated between -UV and +UV surfaces. The MTT assay analysis showed that -UV surface presented significantly higher viability compared to the +UV surface except for one cell population group at 3 h where there were no differences. The nanostructured photoactive TiO 2 surface improved its hydrophilicity by UV irradiation, however no enhancing effect in cell response was confirmed at the time tested compared to the non-irradiated surface.
机译：该研究的目的是表征纳米结构的光敏二氧化钛（TiO 2）涂层，并比较该涂层在紫外线（UV）照射之前和之后人类成骨细胞的细胞反应。将由大约80％的锐钛矿和20％的金红石组成的特定纳米结构TiO 2粉末（Degussa P-25）旋涂到商业纯钛圆片上，然后进行热处理。经过形貌，化学和光催化特性表征后，在紫外线照射之前和之后，将人成骨细胞培养在涂层圆盘上。通过扫描电子显微镜（SEM）评估细胞形态，并通过3-（4,5-二甲基噻唑）-2,5-二苯基四唑溴化物（MTT）分析法分析细胞活力。从接触角分析，紫外线照射后的润湿性显着提高。将培养的细胞用大量拉长的lamellipodia弄平;然而，在-UV和+ UV表面之间没有显示出形态学差异。 MTT分析分析显示，与+ UV表面相比，-UV表面具有明显更高的生存力，除了3 h时没有差异的一组细胞群。纳米结构的光敏性TiO 2表面通过UV辐照提高了其亲水性，但是与未辐照的表面相比，在测试时未确认到细胞反应的增强作用。

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