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首页> 外文期刊>Acta biomaterialia >In vitro cytotoxicity evaluation of porous TiO?-Ag antibacterial coatings for human fetal osteoblasts.
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In vitro cytotoxicity evaluation of porous TiO?-Ag antibacterial coatings for human fetal osteoblasts.

机译：多孔TiO 2 -Ag抗菌涂层对人胎儿成骨细胞的体外细胞毒性评价。

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摘要

Implant-associated infections (IAIs) may be prevented by providing antibacterial properties to the implant surface prior to implantation. Using a plasma electrolytic oxidation (PEO) technique, we produced porous TiO? coatings bearing various concentrations of Ag nanoparticles (Ag NPs) (designated as 0 Ag, 0.3 Ag and 3.0 Ag) on a Ti-6Al-7Nb biomedical alloy. This study investigates the cytotoxicity of these coatings using a human osteoblastic cell line (SV-HFO) and evaluates their bactericidal activity against methicillin-resistant Staphylococcus aureus (MRSA). The release of Ag and the total amount of Ag in the coatings were determined using a graphite furnace atomic absorption spectrometry technique (GF-AAS) and flame-AAS, respectively. Cytotoxicity was evaluated using the AlamarBlue assay coupled with the scanning electron microscopy (SEM) observation of seeded cells and by fluorescence microscopy examination of the actin cytoskeleton and nuclei after 48 h of incubation. Antibacterial activity was assessed quantitatively using a direct contact assay. AlamarBlue viability assay, SEM and fluorescence microscopy observation of the SV-HFO cells showed no toxicity for 0 Ag and 0.3 Ag specimens, after 2, 5 and 7 days of culture, while 3.0 Ag surfaces appeared to be extremely cytotoxic. All Ag-bearing surfaces had good antibacterial activity, whereas Ag-free coatings showed an increase in bacterial numbers. Our results show that the 0.3 Ag coatings offer conditions for optimum cell growth next to antibacterial properties, which makes them extremely useful for the development of new antibacterial dental and orthopedic implants.
机译：通过在植入前为植入物表面提供抗菌特性，可以预防与植入物有关的感染（IAI）。使用等离子电解氧化（PEO）技术，我们生产了多孔TiO2。 Ti-6Al-7Nb生物医学合金上带有各种浓度的Ag纳米颗粒（Ag NPs）（分别表示为0 Ag，0.3 Ag和3.0 Ag）的涂层。这项研究使用人类成骨细胞系（SV-HFO）研究了这些涂层的细胞毒性，并评估了它们对耐甲氧西林金黄色葡萄球菌（MRSA）的杀菌活性。分别使用石墨炉原子吸收光谱技术（GF-AAS）和火焰-AAS确定涂层中Ag的释放量和Ag的总量。孵育48小时后，使用AlamarBlue分析方法结合扫描电子显微镜（SEM）观察接种的细胞，并通过荧光显微镜检查肌动蛋白的细胞骨架和细胞核，评估细胞毒性。使用直接接触测定法定量评估抗菌活性。 SV-HFO细胞的AlamarBlue活力测定，SEM和荧光显微镜观察显示，在培养2天，5天和7天后，0 Ag和0.3 Ag标本没有毒性，而3.0 Ag表面似乎具有极强的细胞毒性。所有含银的表面均具有良好的抗菌活性，而不含银的涂层显示细菌数量增加。我们的结果表明，0.3 Ag涂层可为具有最佳抗菌性能的最佳细胞生长提供条件，这使其对于开发新型抗菌牙科和整形外科植入物极为有用。

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