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首页> 外文期刊>Acta biomaterialia >Hydroxyapatite nanoparticle reinforced peptide amphiphile nanomatrix enhances the osteogenic differentiation of mesenchymal stem cells by compositional ratios.
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Hydroxyapatite nanoparticle reinforced peptide amphiphile nanomatrix enhances the osteogenic differentiation of mesenchymal stem cells by compositional ratios.

机译：羟基磷灰石纳米颗粒增强的肽两亲纳米基质通过组成比增强了间充质干细胞的成骨分化。

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摘要

In the field of bone tissue engineering, there is a need for materials that mimic the native bone extracellular matrix (ECM). This need is met through the creation of biphasic composites intended to mimic both the organic and inorganic facets of the native bone ECM. However, few studies have created composites with organic ECM analogous components capable of directing cellular behaviors and many are not fabricated in the nanoscale. Furthermore, few attempts have been made at investigating how variations of organic and inorganic components affect the osteogenic differentiation of human mesenchymal stem cells (hMSCs). To address these issues, biphasic nanomatrix composites consisting of hydroxyapatite nanoparticles (HANPs) embedded within peptide amphiphile (PA) nanofibers tailored with the RGDS cellular adhesion motif (PA-RGDS) were created at various HANP to PA-RGDS ratios. Fabrication of these biphasic nanomatrix composites was confirmed via scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The long-term cellularity and osteogenic differentiation of hMSCs in response to the different compositional ratios were then assessed by quantifying the timed expression of genes indicative of osteogenic differentiation, alkaline phosphatase activity, and DNA content over time. Decreased cellularity and the expression of genes over time correlated with increasing compositional ratios between HANP and PA-RGDS. The highest HANP to PA-RGDS ratio (66% HANP) exhibited the greatest improvement to the osteogenic differentiation of hMSCs. Overall, these results demonstrate that the compositional ratio of biphasic nanomatrix composites plays an important role in influencing the osteogenic differentiation of hMSCs. Based on the observations presented within this study, these biphasic nanomatrix composites show promise for future usage in bone tissue engineering applications.
机译：在骨组织工程领域中，需要模仿天然骨细胞外基质（ECM）的材料。通过创建旨在模拟天然骨ECM的有机和无机面的双相复合材料，可以满足这种需求。但是，很少有研究创造出具有有机ECM类似成分，能够指导细胞行为的复合材料，而许多研究并未在纳米级制造。此外，在研究有机和无机成分的变化如何影响人间充质干细胞（hMSCs）的成骨分化方面，几乎没有进行任何尝试。为了解决这些问题，以不同的HANP与PA-RGDS比率创建了由羟基磷灰石纳米颗粒（HANP）组成的双相纳米复合材料，该复合材料嵌入了用RGDS细胞粘附基序（PA-RGDS）定制的肽两亲（PA）纳米纤维中。这些双相纳米基质复合材料的制造已通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）确认。然后通过量化指示成骨分化，碱性磷酸酶活性和随时间变化的DNA的基因的定时表达，评估hMSC对不同组成比的长期细胞性和成骨分化。随着时间的流逝，细胞数量的减少和基因的表达与HANP和PA-RGDS之间的组成比增加有关。 HANP与PA-RGDS的比例最高（66％HANP）对hMSC的成骨分化表现出最大的改善。总体而言，这些结果表明，双相纳米基质复合材料的组成比在影响​​hMSC的成骨分化中起着重要作用。基于本研究中提出的观察结果，这些双相纳米基质复合材料显示出有望在骨组织工程应用中的未来用途。

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