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首页> 外文期刊>Acta biomaterialia >BFGF-containing electrospun gelatin scaffolds with controlled nano-architectural features for directed angiogenesis
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BFGF-containing electrospun gelatin scaffolds with controlled nano-architectural features for directed angiogenesis

机译：含BFGF的电纺明胶支架具有受控的纳米结构特征，可用于定向血管生成

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Current therapeutic angiogenesis strategies are focused on the development of biologically responsive scaffolds that can deliver multiple angiogenic cytokines and/or cells in ischemic regions. Herein, we report on a novel electrospinning approach to fabricate cytokine-containing nanofibrous scaffolds with tunable architecture to promote angiogenesis. Fiber diameter and uniformity were controlled by varying the concentration of the polymeric (i.e. gelatin) solution, the feed rate, needle to collector distance, and electric field potential between the collector plate and injection needle. Scaffold fiber orientation (random vs. aligned) was achieved by alternating the polarity of two parallel electrodes placed on the collector plate thus dictating fiber deposition patterns. Basic fibroblast growth factor (bFGF) was physically immobilized within the gelatin scaffolds at variable concentrations and human umbilical vein endothelial cells (HUVEC) were seeded on the top of the scaffolds. Cell proliferation and migration was assessed as a function of growth factor loading and scaffold architecture. HUVECs successfully adhered onto gelatin B scaffolds and cell proliferation was directly proportional to the loading concentrations of the growth factor (0-100 bFGF ng/mL). Fiber orientation had a pronounced effect on cell morphology and orientation. Cells were spread along the fibers of the electrospun scaffolds with the aligned orientation and developed a spindle-like morphology parallel to the scaffold's fibers. In contrast, cells seeded onto the scaffolds with random fiber orientation, did not demonstrate any directionality and appeared to have a rounder shape. Capillary formation (i.e. sprouts length and number of sprouts per bead), assessed in a 3-D in vitro angiogenesis assay, was a function of bFGF loading concentration (0 ng, 50 ng and 100 ng per scaffold) for both types of electrospun scaffolds (i.e. with aligned or random fiber orientation).
机译：当前的治疗性血管生成策略集中在生物反应性支架的开发上，该支架可以在缺血区域中递送多种血管生成性细胞因子和/或细胞。在本文中，我们报告了一种新型的静电纺丝方法，以制备具有可调节结构以促进血管生成的含细胞因子的纳米纤维支架。纤维直径和均匀度是通过改变聚合物（即明胶）溶液的浓度，进料速度，针到收集器的距离以及收集器板和注射针之间的电场电位来控制的。支架纤维的取向（随机与对准）是通过交替放置在收集板上的两个平行电极的极性来实现的，从而决定了纤维的沉积方式。将碱性成纤维细胞生长因子（bFGF）物理固定在浓度可变的明胶支架中，并将人脐静脉内皮细胞（HUVEC）接种在支架顶部。细胞增殖和迁移被评估为生长因子负载和支架结构的函数。 HUVEC成功粘附在明胶B支架上，细胞增殖与生长因子的负载浓度（0-100 bFGF ng / mL）成正比。纤维取向对细胞形态和取向具有显着影响。细胞沿对齐方向沿着电纺支架的纤维散布，并形成与支架纤维平行的纺锤状形态。相反，以随机的纤维取向播种到支架上的细胞没有显示任何方向性，并且看起来具有更圆的形状。在3D体外血管生成测定中评估的毛细血管形成（即每根珠的芽长和芽数）是两种电纺支架的bFGF加载浓度（每个支架0 ng，50 ng和100 ng）的函数。 （即具有对齐或随机的纤维方向）。

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