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首页> 外文期刊>Acta biomaterialia >Functional biopolymer-based matrices for modulation of chronic wound enzyme activities
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Functional biopolymer-based matrices for modulation of chronic wound enzyme activities

机译：基于功能性生物聚合物的基质，可调节慢性伤口酶的活性

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Collagen, collagen/hyaluronic acid (HA) and collagen/HA/chitosan (CS) sponges loaded with epigallocatechin gallate (EGCG), catechin (CAT) and gallic acid (GA) were developed and evaluated as active chronic wound dressings. Their physico-mechanical properties, biostability, biocompatibility and ability to inhibit in vitro myeloperoxidase (MPO) and collagenase - major enzymes related with the persistent inflammation in chronic wounds - were investigated as a function of the biopolymer composition and the polyphenolic compound used. The results demonstrated that the molecular weight of HA influences significantly the bulk properties of the obtained materials: higher elastic modulus, swelling ability and biostability against collagenase were measured when HA with higher molecular weights (830 and 2000 kDa) were added to the collagen matrices. The addition of CS and the polyphenols increased further the biostability of the sponges. Preliminary in vitro tests with fibroblasts revealed that the cells were able to adhere to all sponges. Cell viability was not affected significantly by the addition of the polyphenols; however, the presence of CS or high molecular weight HA in the sponge composition was associated with lower cellular viability. Finally, all specimens containing polyphenols efficiently inhibited the MPO activity. The highest inhibition capacity was observed for EGCG (IC50 = 15 ?? 1 ??M) and it was coupled to the highest extent of binding to the biopolymers (80%) and optimal release profile from the sponges that allowed for prolonged (up to 3-5 days) effects. ? 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
机译：开发了装载表没食子儿茶素没食子酸酯（EGCG），儿茶素（CAT）和没食子酸（GA）的胶原蛋白，胶原蛋白/透明质酸（HA）和胶原蛋白/ HA /壳聚糖（CS）海绵，并将其评估为活性的慢性伤口敷料。研究了它们的物理机械性能，生物稳定性，生物相容性以及抑制体外髓过氧化物酶（MPO）和胶原酶的能力-与慢性伤口持续发炎有关的主要酶-与生物聚合物组成和所用多酚化合物的关系。结果表明，HA的分子量显着影响所得材料的整体性能：将较高分子量（830和2000 kDa）的HA添加到胶原蛋白基质中时，可测得更高的弹性模量，溶胀能力和对胶原酶的生物稳定性。 CS和多酚的添加进一步提高了海绵的生物稳定性。用成纤维细胞进行的初步体外测试表明，这些细胞能够粘附在所有海绵上。添加多酚对细胞活力没有显着影响。然而，海绵组合物中CS或高分子量HA的存在与较低的细胞活力有关。最后，所有含有多酚的标本均有效抑制了MPO活性。观察到对EGCG的最高抑制能力（IC50 = 15 ?? 1 ?? M），它与生物聚合物的最大结合程度（> 80％）和最佳的从海绵中释放的特性相结合，可以延长（向上）至3-5天）效果。 ？ 2012年Acta Materialia Inc.由Elsevier Ltd.发行。保留所有权利。

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