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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Novel quartz crystal microbalance based biosensor for detection of oral epithelial cell-microparticle interaction in real-time
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Novel quartz crystal microbalance based biosensor for detection of oral epithelial cell-microparticle interaction in real-time

机译：基于新型石英晶体微天平的生物传感器可实时检测口腔上皮细胞-微粒相互作用

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摘要

Recent applications of quartz crystal resonant sensor technology to monitor cell adhesion and specific ligand interaction processes has triggered the development of a new category of quartz crystal microbalance (QCM) based biosensors. In this study human oral epithelial cells (H376) were cultured on quartz sensors and their response to microspheres investigated in situ using the QCM technique. The results demonstrated that this novel biosensor was able to follow cell-microsphere interactions in real-time and under conditions of flow as would occur in the oral cavity. Unique frequency profiles generated in response to the microspheres were postulated to be due to phases of mass addition and altered cellular rigidity. supporting microscopic evidence demonstrated that the unique frequency responses obtained to these interactions were in part due to binding between the cell surface and the microspheres. Furthermore, a cellular uptake process, in response to microsphere loading was identified and this, by influencing the rigidity of the cellular cytoskeleton, was also detectable through the frequency responses obtained. (c) 2007 Elsevier B.V. All rights reserved.
机译：石英晶体共振传感器技术在监测细胞粘附和特定配体相互作用过程中的最新应用触发了基于石英晶体微天平（QCM）的新型生物传感器的开发。在这项研究中，人类口腔上皮细胞（H376）在石英传感器上培养，并使用QCM技术对它们对微球的反应进行了原位研究。结果表明，这种新型生物传感器能够实时并在口腔内流动的情况下跟踪细胞-微球相互作用。假定响应于微球而产生的独特频率分布是由于质量增加和细胞刚性改变的阶段所致。支持的微观证据表明，对这些相互作用获得的独特频率响应部分归因于细胞表面与微球之间的结合。此外，已经确定了响应微球负载的细胞摄取过程，并且通过影响细胞骨架的刚度，还可以通过获得的频率响应来检测该过程。 （c）2007 Elsevier B.V.保留所有权利。

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