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首页> 外文期刊>Acta biomaterialia >A tunable synthetic hydrogel system for culture of retinal ganglion cells and amacrine cells.
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A tunable synthetic hydrogel system for culture of retinal ganglion cells and amacrine cells.

机译：用于培养视网膜神经节细胞和无长突细胞的可调合成水凝胶系统。

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摘要

The central nervous system consists of complex groups of individual cells that receive electrical, chemical and physical signals from their local environment. Standard in vitro cell culture methods rely on two-dimensional (2-D) substrates that poorly simulate in vivo neural architecture. Neural cells grown in three-dimensional (3-D) culture systems may provide an opportunity to study more accurate representations of the in vivo environment than 2-D cultures. Furthermore, each specific type of neuron depends on discrete compositions and physical properties of their local environment. Previously, we developed a library of hydrogels composed of poly(ethylene glycol) and poly(l-lysine) which exhibit a wide range of mechanical properties. Here, we identified specific scaffolds from this library that readily support the survival, migration and neurite outgrowth of purified retinal ganglion cells and amacrine cells. These data address important biological questions about the interaction of neurons with the physical and chemical properties of their local environment and provide further insight for engineering neural tissue for cell-replacement therapies following injury.
机译：中枢神经系统由单个细胞的复杂组组成，这些细胞从其本地环境接收电，化学和物理信号。标准的体外细胞培养方法依赖于不能很好地模拟体内神经结构的二维（2-D）底物。与二维培养相比，在三维（3-D）培养系统中生长的神经细胞可能提供了研究体内环境更精确表示的机会。此外，每种特定类型的神经元都取决于其局部环境的离散组成和物理特性。以前，我们开发了由聚（乙二醇）和聚（1-赖氨酸）组成的水凝胶库，它们具有广泛的机械性能。在这里，我们从该文库中鉴定了特定的支架，这些支架容易支持纯化的视网膜神经节细胞和无长突细胞的存活，迁移和神经突向外生长。这些数据解决了有关神经元与其局部环境的物理和化学特性相互作用的重要生物学问题，并为工程神经组织损伤后的细胞置换疗法提供了进一步的见识。

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