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首页> 外文期刊>Acta biomaterialia >Formation and stability of interpenetrating polymer network hydrogels consisting of fibrin and hyaluronic acid for tissue engineering
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Formation and stability of interpenetrating polymer network hydrogels consisting of fibrin and hyaluronic acid for tissue engineering

机译：由纤维蛋白和透明质酸组成的互穿聚合物网络水凝胶的形成和稳定性

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摘要

Fibrin gel is widely used as a tissue engineering scaffold. However, it has poor mechanical properties, which often result in rapid contraction and degradation of the scaffold. An interpenetrating polymer network (IPN) hydrogel composed of fibrin and hyaluronic acid-tyramine (HA-Tyr) was developed to improve the mechanical properties. The fibrin network was formed by cleaving fibrinogen with thrombin, producing fibrin monomers that rapidly polymerize. The HA network was formed through the coupling of tyramine moieties using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The degree of crosslinking of the HA-Tyr network can be tuned by varying the H2O2 concentration, producing IPN hydrogels with different storage moduli (G??). While fibrin gels were completely degraded in the presence of plasmin and contracted when embedded with cells, the shape of the IPN hydrogels was maintained due to structural support by the HA-Tyr networks. Cell proliferation and capillary formation occurred in IPN hydrogels and were found to decrease with increasing G?? of the hydrogels. The results suggest that fibrin-HA-Tyr IPN hydrogels are a potential alternative to fibrin gels as scaffolds for tissue engineering applications that require shape stability. ? 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
机译：纤维蛋白凝胶被广泛用作组织工程支架。然而，它具有差的机械性能，这常常导致支架的快速收缩和降解。开发了由纤维蛋白和透明质酸-酪胺（HA-Tyr）组成的互穿聚合物网络（IPN）水凝胶，以改善机械性能。血纤蛋白网络是通过将血纤蛋白原与凝血酶裂解而形成的，从而产生迅速聚合的血纤蛋白单体。 HA网络是通过使用辣根过氧化物酶（HRP）和过氧化氢（H2O2）偶联酪胺部分而形成的。可以通过改变H 2 O 2的浓度来调节HA-Tyr网络的交联度，从而生产出具有不同存储模量（G 10）的IPN水凝胶。尽管纤维蛋白凝胶在纤溶酶存在下会完全降解，并在包埋细胞时收缩，但由于HA-Tyr网络的结构支持，IPN水凝胶的形状得以保持。在IPN水凝胶中发生细胞增殖和毛细血管形成，并发现其随Gβ增加而降低。水凝胶。结果表明，纤维蛋白-HA-Tyr IPN水凝胶是纤维蛋白凝胶的潜在替代品，可作为需要形状稳定性的组织工程应用的支架。 ？ 2012年Acta Materialia Inc.由Elsevier Ltd.发行。保留所有权利。

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