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首页> 外文期刊>Acta biomaterialia >Size effect of amphiphilic poly(γ-glutamic acid) nanoparticles on cellular uptake and maturation of dendritic cells in vivo
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Size effect of amphiphilic poly(γ-glutamic acid) nanoparticles on cellular uptake and maturation of dendritic cells in vivo

机译：两亲性聚（γ-谷氨酸）纳米粒子对体内树突状细胞摄取和成熟的大小影响

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摘要

We prepared size-regulated nanoparticles (NPs) composed of amphiphilic poly(γ-glutamic acid) (γ-PGA). In this study, 40, 100 and 200 nm γ-PGA-graft-l-phenylalanine ethylester (γ-PGA-Phe) NPs were employed. The size of NPs significantly influenced the uptake and activation behaviors of antigen-presenting cells (APCs). When 40 nm γ-PGA-Phe NPs were applied to these cells in vitro, they were highly activated compared with 100 and 200 nm NPs, while cellular uptake was size dependent. The size of the γ-PGA-Phe NPs also significantly affected their migration to the lymph nodes and uptake behavior of NPs by dendritic cells (DCs) in vivo. The 40 nm γ-PGA-Phe NPs migrated more rapidly to the lymph nodes and were taken up by a greater number of DCs compared with 100 and 200 nm NPs. On the other hand, when the amount of γ-PGA-Phe NPs taken up per DC was evaluated, it was higher for 100 and 200 nm NPs than for 40 nm NPs, which suggests that the larger γ-PGA-Phe NPs can deliver a large amount of antigen to a single DC compared with smaller NPs. Furthermore, when examined the maturation of DCs in lymph nodes, 40 nm γ-PGA-Phe NPs efficiently stimulated DCs. These results suggest that the activation, uptake behavior by APCs, migration to lymph nodes, and DC maturation can be controlled by the size of γ-PGA-Phe NPs.
机译：我们准备了由两亲性聚（γ-谷氨酸）（γ-PGA）组成的尺寸可调节的纳米颗粒（NPs）。在这项研究中，使用了40、100和200 nm的γ-PGA-接枝-1-苯丙氨酸乙酯（γ-PGA-Phe）NP。 NP的大小显着影响抗原呈递细胞（APC）的摄取和激活行为。当在体外将40 nmγ-PGA-PheNP应用于这些细胞时，与100和200 nm NP相比，它们被高度激活，而细胞摄取则取决于大小。 γ-PGA-PheNP的大小也显着影响其向淋巴结的迁移以及体内树突状细胞（DC）对NP的吸收行为。与100和200 nm NP相比，40 nmγ-PGA-PheNP迁移到淋巴结的速度更快，并被更多的DC吸收。另一方面，当评估每个DC吸收的γ-PGA-PheNP数量时，其100和200 nm NP的数量要高于40 nm NP，这表明较大的γ-PGA-PheNP可以递送。与较小的NP相比，单个DC需要大量抗原。此外，当检查淋巴结中DC的成熟时，40 nmγ-PGA-PheNP可以有效刺激DC。这些结果表明，γ-PGA-PheNP的大小可以控制APC的激活，摄取行为，向淋巴结的迁移和DC成熟。

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