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首页> 外文期刊>Acta biomaterialia >The influence of biomimetic topographical features and the extracellular matrix peptide RGD on human corneal epithelial contact guidance
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The influence of biomimetic topographical features and the extracellular matrix peptide RGD on human corneal epithelial contact guidance

机译：仿生地形特征和细胞外基质肽RGD对人角膜上皮接触指导的影响

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A major focus in the field of tissue engineering is the regulation of essential cell behaviors through biophysical and biochemical cues from the local extracellular environment. The impact of nanotopographical cues on human corneal epithelial cell (HCEC) contact guidance, proliferation, migration and adhesion have previously been demonstrated. In the current report we have expanded our study of HCEC responses to include both biophysical and controlled biochemical extracellular cues. By exploiting methods for the layer-by-layer coating of substrates with reactive poly(ethylene imine)/poly(2-vinyl-4,4- dimethylazlactone)-based multilayer thin films we have incorporated a single adhesion peptide motif, Arg-Gly-Asp (RGD), on topographically patterned substrates. This strategy eliminates protein adsorption onto the surface, thus decoupling the effects of the HCEC response to topographical cues from adsorbed proteins and soluble media proteins. The direction of cell alignment was dependent on the scale of the topographical cues and, to less of an extent, the culture medium. In EpiLife? medium cell alignment to unmodified-NOA81 topographical features, which allowed protein adsorption, differed significantly from cell alignment on RGD-modified features. These results demonstrate that the surface chemical composition significantly affects how HCECs respond to topographical cues. In summary, we have demonstrated modulation of the HCEC response to environmental cues through critical substrate and soluble parameters. ? 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
机译：组织工程领域的主要焦点是通过来自局部细胞外环境的生物物理和生化线索来调节基本细胞行为。先前已经证明了纳米地形学线索对人角膜上皮细胞（HCEC）接触指导，增殖，迁移和粘附的影响。在本报告中，我们已扩展了对HCEC反应的研究，以涵盖生物物理和受控的生化细胞外信号。通过利用基于反应性聚（乙烯亚胺）/聚（2-乙烯基-4,4-二甲基氮杂内酯）的多层薄膜对基材进行逐层涂覆的方法，我们引入了单个粘附肽基序Arg-Gly -Asp（RGD），在已构图的基板上。该策略消除了蛋白质吸附到表面上的情况，从而将HCEC响应对地形线索的影响从吸附的蛋白质和可溶性介质蛋白质中分离出来。细胞排列的方向取决于地形线索的规模，并且在较小程度上取决于培养基。在EpiLife中？中等细胞与未经修饰的NOA81地形特征的对齐方式（允许蛋白质吸附）与RGD修饰的特征上的细胞对齐方式有显着差异。这些结果表明，表面化学成分显着影响HCEC对地形线索的反应。总而言之，我们已经证明了HCEC通过关键底物和可溶性参数对环境线索的响应的调节。 ？ 2012年Acta Materialia Inc.由Elsevier Ltd.发行。保留所有权利。

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