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首页> 外文期刊>Acta biomaterialia >Fibrous scaffolds loaded with protein prepared by blend or coaxial electrospinning.
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Fibrous scaffolds loaded with protein prepared by blend or coaxial electrospinning.

机译：通过共混或同轴静电纺丝制备的装有蛋白质的纤维状支架。

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摘要

The aim of the present study was to fabricate polycaprolactone-based nanofibrous scaffolds with incorporated protein via either the blend or coaxial electrospinning technique. Both techniques were compared with respect to processing set-up and scaffold characteristics as well as the release kinetics and biological activity of the loaded protein. Bovine serum albumin was used as a model protein to determine release profiles, while alkaline phosphatase was used to determine protein activity after the electrospinning process. Coaxial electrospinning resulted in a uniform fiber morphology with a core-shell structure, and a homogeneous protein distribution throughout the core of the fibers. In contrast, blend electrospinning formed bead-like fibers with a heterogeneous protein distribution in the fibers. The coaxial scaffold exhibited more sustained release profiles than the comparative blend scaffold, and the additive poly(ethylene glycol) (PEG) in the coaxial scaffold accelerated protein release. Both electrospinning processes decreased the biological activity of the incorporated protein, but coaxial electrospinning with PEG as an additive showed up to 75% preservation of the initial biological activity. Thus, coaxial electrospinning was demonstrated to be superior to blend electrospinning for the preparation of nanofibrous scaffolds with a uniform fibrous structure and protein distribution and sustained protein release kinetics as well as high preservation of the protein activity.
机译：本研究的目的是通过掺混或同轴静电纺丝技术制造掺入蛋白质的聚己内酯基纳米纤维支架。比较了两种技术的加工结构和支架特性，以及所装载蛋白质的释放动力学和生物活性。牛血清白蛋白用作模型蛋白质以确定释放曲线，而碱性磷酸酶用于确定静电纺丝过程后的蛋白质活性。同轴电纺丝产生具有核-壳结构的均匀纤维形态，以及遍布整个纤维核的均匀蛋白质分布。相反，共混纺丝形成的珠状纤维在纤维中具有不均匀的蛋白质分布。同轴支架比对比共混支架表现出更多的持续释放特性，并且同轴支架中的添加剂聚（乙二醇）（PEG）促进了蛋白质的释放。两种静电纺丝工艺均降低了所掺入蛋白质的生物活性，但以PEG作为添加剂的同轴静电纺丝可保留高达75％的初始生物活性。因此，对于制备具有均匀纤维结构和蛋白质分布以及持续的蛋白质释放动力学以及高度保留蛋白质活性的纳米纤维支架，同轴电纺被证明优于混合电纺。

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