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首页> 外文期刊>Acta biomaterialia >The effect of type II collagen coating of chitosan fibrous scaffolds on mesenchymal stem cell adhesion and chondrogenesis.
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The effect of type II collagen coating of chitosan fibrous scaffolds on mesenchymal stem cell adhesion and chondrogenesis.

机译：壳聚糖纤维支架的II型胶原蛋白涂层对间充质干细胞粘附和软骨形成的影响。

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The biocompatibility of chitosan and its similarity to glycosaminoglycans (GAG) make it attractive for cartilage tissue engineering. We have previously reported improved chondrogenesis but limited cell adhesion on chitosan scaffolds. Our objectives were to produce chitosan scaffolds coated with different densities of type II collagen and to evaluate the effect of this coating on mesenchymal stem cell (MSC) adhesion and chondrogenesis. Chitosan fibrous scaffolds were obtained by a wet spinning method and coated with type II collagen at two different densities. A polyglycolic acid mesh served as a reference group. The scaffolds were characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and type II collagen content. Constructs were analyzed after MSCs seeding via live/dead assay, weight and DNA evaluations, SEM, and TEM. Constructs were cultured in chondrogenic medium for 21 days prior to quantitative analysis (weight, DNA, and GAG), SEM, TEM, histology, immunohistochemistry, and quantitative real time polymerase chain reaction. The cell attachment and distribution after seeding correlated with the density of type II collagen. The cell number, the matrix production, and the expression of genes specific for chondrogenesis were improved after culture in collagen coated chitosan constructs. These findings encourage the use of type II collagen for coating chitosan scaffolds to improve MSCs adhesion and chondrogenesis, and confirm the importance of biomimetic scaffolds for tissue engineering.
机译：壳聚糖的生物相容性及其与糖胺聚糖（GAG）的相似性使其对软骨组织工程具有吸引力。先前我们已经报道了软骨形成的改善，但是壳聚糖支架上的细胞粘附受到限制。我们的目标是生产包覆有不同密度II型胶原蛋白的壳聚糖支架，并评估该涂层对间充质干细胞（MSC）粘附和软骨形成的影响。通过湿纺法获得壳聚糖纤维支架，并以两种不同的密度涂覆II型胶原。聚乙醇酸网用作参考组。通过傅立叶变换红外光谱，扫描电子显微镜（SEM），透射电子显微镜（TEM）和II型胶原含量表征支架。在MSCs播种后，通过活/死分析，重量和DNA评估，SEM和TEM分析构建体。在定量分析（重量，DNA和GAG），SEM，TEM，组织学，免疫组织化学和定量实时聚合酶链反应之前，将构建体在软骨形成培养基中培养21天。接种后细胞的附着和分布与Ⅱ型胶原的密度有关。在胶原蛋白包被的壳聚糖构建物中培养后，细胞数目，基质产生和软骨形成特异性基因的表达得到改善。这些发现鼓励使用II型胶原蛋白涂覆壳聚糖支架以改善MSC的粘附和软骨形成，并证实了仿生支架在组织工程中的重要性。

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