• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Protein adhesion and cell response on atmospheric pressure dielectric barrier discharge-modified polymer surfaces
【24h】

Protein adhesion and cell response on atmospheric pressure dielectric barrier discharge-modified polymer surfaces

机译：大气压介质阻挡放电改性聚合物表面上的蛋白质粘附和细胞反应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gaseous plasma discharges are one of the most common means to modify the surface of a polymer without affecting its bulk properties. However, this normally requires the materials to be processed in vacuo to create the active species required to permanently modify the surface chemistry. The ability to invoke such changes under normal ambient conditions in a cost-effective manner has much to offer to enhance the response of medical implants in vivo. It is therefore important to accurately determine the nature and scale of the effects derived from this technology. This paper reports on the modification of poly(styrene) (PS) and poly(methyl methacrylate) (PMMA) using atmospheric pressure plasma processing via exposure to a dielectric barrier discharge (DBD). The changes in surface chemistry and topography after DBD treatment were characterised using water contact angle, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy. A marked increase in the surface oxygen concentration was observed for both PMMA and PS. An increase in surface roughness was observed for PMMA, but not for PS. These changes were found to result in an increase in surface wettability for both polymers. Adsorption of albumin (Alb) onto these substrates was studied using XPS and quartz crystal microbalance with dissipation (QCM-D). The rate of adsorption of Alb onto pristine PMMA and PS was faster than that on the DBD-treated polymers. XPS indicated that a similar concentration of Alb occurred on both of the treated surfaces. Deconvolution of the Cls XPS spectra showed that Alb is adsorbed differently on pristine (hydrophobic) compared to DBD-treated (hydrophilic) surfaces, with more polar functional groups oriented towards the upper surface in the latter case. The QCM-D data corroborates this finding, in that a more viscoelastic layer of Alb was formed on the DBD-treated surfaces relative to that on the pristine surfaces. It was also found that Alb was more easily replaced by larger proteins from foetal bovine serum on the DBD-treated surfaces. The viability of human lens epithelial cells on both of the DBD-treated polymer surface was significantly (P< 0.05) greater than on the respective pristine surfaces. In addition, cells that adhered to the treated polymers exhibited a polygonal morphology with well spread actin stress fibres compared with the contracted shape displayed on the pristine surfaces. The results presented here clearly indicate that DBD surface modification has the capability to influence key protein and cell responses.
机译：气态等离子体放电是改性聚合物表面而不影响其本体性能的最常用方法之一。然而，这通常需要将材料真空处理以产生永久改变表面化学性质所需的活性物质。在正常环境条件下以具有成本效益的方式调用此类更改的功能可为增强体内医疗植入物的响应提供很多帮助。因此，准确确定从该技术获得的效果的性质和规模非常重要。本文报道了通过暴露于介电势垒放电（DBD）的大气压等离子体处理对聚苯乙烯（PS）和聚甲基丙烯酸甲酯（PMMA）的改性。使用水接触角，X射线光电子能谱（XPS）和原子力显微镜观察了DBD处理后表面化学和形貌的变化。对于PMMA和PS，均观察到表面氧浓度的显着增加。对于PMMA，观察到表面粗糙度增加，而对于PS，则未观察到。发现这些变化导致两种聚合物的表面润湿性增加。使用XPS和带耗散的石英晶体微量天平（QCM-D）研究了白蛋白（Alb）在这些基质上的吸附。 Alb吸附在原始PMMA和PS上的速率比DBD处理的聚合物快。 XPS表明在两个处理过的表面上都发生了相似浓度的Alb。 Cls XPS光谱的反卷积表明，与DBD处理（亲水）表面相比，Alb在原始（疏水）表面的吸附方式有所不同，在后一种情况下，极性较大的官能团朝向上表面。 QCM-D数据证实了这一发现，因为与原始表面相比，在DBD处理过的表面上形成了更具粘弹性的Alb层。还发现，在DBD处理过的表面上，胎牛血清中的较大蛋白质更容易替代Alb。在经过DBD处理的两个聚合物表面上，人晶状体上皮细胞的活力均显着（P <0.05），高于各个原始表面上的活力。另外，与原始表面上显示的收缩形状相比，粘附到处理过的聚合物上的细胞表现出具有广泛分布的肌动蛋白应力纤维的多边形形态。此处给出的结果清楚地表明，DBD表面修饰具有影响关键蛋白质和细胞反应的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号