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首页> 外文期刊>Acta biomaterialia >In vitro biocompatibility of poly(vinylidene fluoride-trifluoroethylene)/barium titanate composite using cultures of human periodontal ligament fibroblasts and keratinocytes.
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In vitro biocompatibility of poly(vinylidene fluoride-trifluoroethylene)/barium titanate composite using cultures of human periodontal ligament fibroblasts and keratinocytes.

机译：聚（偏二氟乙烯-三氟乙烯）/钛酸钡复合材料的体外生物相容性，使用人牙周膜成纤维细胞和角质形成细胞培养。

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摘要

The aim of this work was to evaluate the biocompatibility of poly(vinylidene fluoride-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT) membrane to be used in guided tissue regeneration (GTR). Fibroblasts from human periodontal ligament (hPDLF) and keratinocytes (SCC9) were plated on P(VDF-TrFE)/BT and polytetrafluorethylene membranes at a cell density of 20,000 cells well(-1) and cultured for up to 21 days. Cell morphology, adhesion and proliferation were evaluated in hPDLF and keratinocytes, while total protein content and alkaline phosphatase (ALP) activity were assayed only for hPDLF. Using a higher cell density, real-time polymerase chain reaction (PCR) was performed to assess the expression of typical genes of hPDLF, such as periostin, PDLs17, S100A4 and fibromodulin, and key phenotypic markers of keratinocytes, including involucrin, keratins 1, 10 and 14. Expression of the apoptotic genes bax, bcl-2 and survivin was evaluated for both cultures. hPDLF adhered and spread more on P(VDF-TrFE)/BT, whereas keratinocytes showed a round shape on both membranes. hPDLF adhesion was greater on P(VDF-TrFE)/BT at 2 and 4h, while keratinocyte adhesion was similar for both membranes. Whereas proliferation was significantly higher for hPDLF on P(VDF-TrFE)/BT at days 1 and 7, no signs of keratinocyte proliferation could be noticed for both membranes. Total protein content was greater on P(VDF-TrFE)/BT at 7, 14 and 21 days, and higher levels of ALP activity were observed on P(VDF-TrFE)/BT at 21 days. Real-time PCR revealed higher expression of phenotypic markers of hPDLF and keratinocytes as well as greater expression of apoptotic genes in cultures grown on P(VDF-TrFE)/BT. These results indicate that, by favoring hPDLF adhesion, spreading, proliferation and typical mRNA expression, P(VDF-TrFE)/BT membrane should be considered an advantageous alternative for GTR.
机译：这项工作的目的是评估聚（偏二氟乙烯-三氟乙烯）/钛酸钡（P（VDF-TrFE）/ BT）膜的生物相容性，以用于引导组织再生（GTR）。将人牙周膜（hPDLF）和角质形成细胞（SCC9）的成纤维细胞以20,000个细胞well（-1）的细胞密度接种于P（VDF-TrFE）/ BT和聚四氟乙烯膜上，并培养长达21天。在hPDLF和角质形成细胞中评估了细胞形态，粘附和增殖，而仅对hPDLF进行了总蛋白含量和碱性磷酸酶（ALP）活性的测定。使用较高的细胞密度，进行了实时聚合酶链反应（PCR），以评估hPDLF的典型基因（如骨膜素，PDLs17，S100A4和纤维调节蛋白）的表达，以及角质形成细胞的关键表型标记，包括整合素，角蛋白1参见图10和14。评估了两种培养物的凋亡基因bax，bcl-2和survivin的表达。 hPDLF粘附并更多地散布在P（VDF-TrFE）/ BT上，而角质形成细胞在两个膜上均呈圆形。在2h和4h，hPDLF在P（VDF-TrFE）/ BT上的粘附力更大，而两个膜的角质形成细胞粘附力相似。尽管在第1天和第7天，hPDLF在P（VDF-TrFE）/ BT上的增殖明显更高，但是在两个膜上都没有发现角质形成细胞增殖的迹象。在第7、14和21天，P（VDF-TrFE）/ BT的总蛋白含量更高，而在第21天，P（VDF-TrFE）/ BT的总蛋白含量更高。实时PCR显示，在P（VDF-TrFE）/ BT上培养的培养物中，hPDLF和角质形成细胞的表型标记物表达更高，并且凋亡基因的表达更高。这些结果表明，通过促进hPDLF的粘附，扩散，增殖和典型的mRNA表达，P（VDF-TrFE）/ BT膜应被认为是GTR的有利替代物。

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