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Surface modification of and selective protein attachment to a flexible microarray pattern using atmospheric plasma with a reactive gas.

机译:使用大气等离子体和反应气体对柔性微阵列图案进行表面修饰和选择性蛋白质附着。

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摘要

The hydrophobic and hydrophilic properties of the surface of poly-ether sulfone (PES) films were controlled by an atmospheric pressure plasma (AP) treatment using reactive gases (Ar/H(2) and Ar/O(2)). The surface properties of the Ar/H(2) and Ar/O(2) in series AP-treated PES films showed higher surface roughness (approximately 120%), surface energy (approximately 30%) and hydrophilic properties (oxygen content approximately 10%) than the Ar/O(2) AP-treated PES film. The protein staining results confirmed that an activated region on the patterned PES film with high selectivity and sensitivity was well-defined and formed. This method is suitable for fabricating flexible protein adhesive chips with uniform biomolecular adhesive properties.
机译:聚醚砜(PES)膜表面的疏水性和亲水性通过使用反应气体(Ar / H(2)和Ar / O(2))的大气压等离子体(AP)处理来控制。连续AP处理的PES膜中Ar / H(2)和Ar / O(2)的表面性能显示出较高的表面粗糙度(约120%),表面能(约30%)和亲水性(氧含量约10) %)比Ar / O(2)AP处理的PES膜高。蛋白质染色结果证实,在图案化的PES膜上具有高选择性和灵敏度的活化区被明确定义并形成。该方法适用于制造具有均匀生物分子粘合性能的柔性蛋白质粘合芯片。

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