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首页> 外文期刊>Acta biomaterialia >Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds.
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Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds.

机译：电纺玉米蛋白纤维的细胞相容性交联，用于开发水稳定性组织工程支架。

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This paper reports a new method of cross-linking electrospun zein fibers using citric acid as a non-toxic cross-linker to enhance the water stability and cytocompatibility of zein fibers for tissue engineering and other medical applications. The electrospun structure has many advantages over other types of structures and protein-based biomaterials possess unique properties preferred for tissue engineering and other medical applications. However, ultrafine fiber matrices developed from proteins have poor mechanical properties and morphological stability in the aqueous environments required for medical applications. Efforts have been made to improve the water stability of electrospun protein scaffolds using cross-linking and other approaches, but the current methods have major limitations, such as cytotoxicity and low efficiency. In this research electrospun zein fibers were cross-linked with citric acid without using any toxic catalysts. The stability of the cross-linked fibers in phosphate-buffered saline and their ability to support the attachment, spreading and proliferation of mouse fibroblast cells were studied. The cross-linked electrospun fibers retained their ultrafine fibrous structure even after immersion in PBS at 37 degrees C for up to 15 days. Citric acid cross-linked electrospun zein scaffolds showed better attachment, spreading and proliferation of fibroblast cells than uncross-linked electrospun zein fibers, cross-linked zein films and electrospun polylactide fibers.
机译：本文报道了一种新的交联静电纺丝玉米蛋白纤维的方法，该方法使用柠檬酸作为无毒交联剂来增强玉米蛋白纤维的水稳定性和细胞相容性，以用于组织工程和其他医学应用。与其他类型的结构相比，电纺结构具有许多优势，基于蛋白质的生物材料具有独特的特性，这些特性是组织工程和其他医学应用的首选。然而，由蛋白质形成的超细纤维基质在医学应用所需的水性环境中具有较差的机械性能和形态稳定性。已经努力使用交联和其他方法来改善电纺蛋白支架的水稳定性，但是当前的方法具有主要的局限性，例如细胞毒性和低效率。在这项研究中，电纺玉米蛋白纤维与柠檬酸交联，而无需使用任何有毒的催化剂。研究了交联纤维在磷酸盐缓冲盐水中的稳定性及其支持小鼠成纤维细胞附着，扩散和增殖的能力。交联的电纺纤维即使在37°C的PBS中浸泡长达15天后仍保持其超细纤维结构。柠檬酸交联的电纺玉米醇溶蛋白支架比未交联的电纺玉米蛋白纤维，交联的玉米蛋白膜和电纺聚丙交酯纤维表现出更好的附着，扩散和成纤维细胞增殖。

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