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首页> 外文期刊>Acta biomaterialia >Validation of a high-throughput methodology to assess the effects of biomaterials on dendritic cell phenotype
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Validation of a high-throughput methodology to assess the effects of biomaterials on dendritic cell phenotype

机译：验证高通量方法以评估生物材料对树突状细胞表型的影响

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摘要

A variety of combination products composed of biomaterials and biologies have been developed for tissue regeneration or vaccine delivery. The host immune response to the immunogenic biological components in such products may be modulated by the biomaterial component. Distinct biomaterials have been shown to differentially affect the maturation of dendritic cells (DCs). DCs are professional antigen-presenting cells (APCs) that bridge innate and adaptive immunity and play a central role in inducing immunity or initiating immune tolerance. However, the biomaterials systems used to study DC response thus far have been insufficient to draw a clear conclusion as to which biomaterial properties are the key to controlling DC phenotype. In this study, we developed a 96-well filter plate-based high-throughput (HTP) methodology to assess DC maturation upon biomaterial treatment. Equivalent biomaterial effects on DC phenotype were measured using the conventional flow cytometric and filter-plate method, which validated the HTP methodology. This methodology will be used to screen a large number of biomaterials simultaneously and to draw correlations between material properties and DC phenotype, thereby providing biomaterial design criteria and immunomodulatory strategies for both tissue engineering and vaccine delivery applications.
机译：已经开发了由生物材料和生物组成的多种组合产品，用于组织再生或疫苗递送。宿主对此类产品中免疫原性生物成分的免疫反应可以通过生物材料成分来调节。不同的生物材料已显示出差异地影响树突状细胞（DC）的成熟。 DC是专业的抗原呈递细胞（APC），可桥接先天性和适应性免疫，并在诱导免疫或启动免疫耐受中起核心作用。然而，迄今为止，用于研究DC反应的生物材料系统还不足以得出明确的结论，即哪些生物材料特性是控制DC表型的关键。在这项研究中，我们开发了一种基于96孔滤板的高通量（HTP）方法，以评估生物材料处理后DC的成熟度。使用常规流式细胞仪和滤板法测量了等效的生物材料对DC表型的影响，该方法验证了HTP方法学的有效性。该方法将用于同时筛选大量生物材料，并得出材料特性与DC表型之间的相关性，从而为组织工程和疫苗输送应用提供生物材料设计标准和免疫调节策略。

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