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首页> 外文期刊>Acta biomaterialia >In vitro cellular response and in vivo primary osteointegration of electrochemically modified titanium.
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In vitro cellular response and in vivo primary osteointegration of electrochemically modified titanium.

机译：电化学修饰钛的体外细胞反应和体内初级骨整合。

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摘要

Anodic spark deposition (ASD) is an attractive technique for improving the implant-bone interface that can be applied to titanium and titanium alloys. This technique produces a surface with microporous morphology and an oxide layer enriched with calcium and phosphorus. The aim of the present study was to investigate the biological response in vitro using primary human osteoblasts as a cellular model and the osteogenic primary response in vivo within a short experimental time frame (2 and 4 weeks) in an animal model (rabbit). Responses were assessed by comparing the new electrochemical biomimetic treatments to an acid-etching treatment as control. The in vitro biological response was characterized by cell morphology, adhesion, proliferation activity and cell metabolic activity. A complete assessment of osteogenic activity in vivo was achieved by estimating static and dynamic histomorphometric parameters at several time points within the considered time frame. The in vitro study showed enhanced osteoblast adhesion and higher metabolic activity for the ASD-treated surfaces during the first days after seeding compared to the control titanium. For the ASD surfaces, the histomorphometry indicated a higher mineral apposition rate within 2 weeks and a more extended bone activation within the first week after surgery, leading to more extensive bone-implant contact after 2 weeks. In conclusion, the ASD surface treatments enhanced the biological response in vitro, promoting an early osteoblast adhesion, and the osteointegrative properties in vivo, accelerating the primary osteogenic response.
机译：阳极火花沉积（ASD）是用于改善可应用于钛和钛合金的植入物-骨界面的一种有吸引力的技术。该技术产生具有微孔形态的表面和富含钙和磷的氧化物层。本研究的目的是在动物模型（兔）的短实验时间内（2周和4周），使用原代人成骨细胞作为细胞模型研究体外生物学反应，以及体内成骨原发反应。通过将新的电化学仿生处理与酸蚀处理作为对照进行比较，从而评估响应。体外生物学应答的特征在于细胞形态，粘附，增殖活性和细胞代谢活性。通过估算所考虑时间范围内几个时间点的静态和动态组织形态计量学参数，可以实现对体内成骨活性的完整评估。体外研究表明，与对照钛相比，播种后第一天ASD处理的表面具有增强的成骨细胞粘附性和更高的代谢活性。对于ASD表面，组织形态测量表明，术后2周内矿物质沉积率更高，而在术后第一周内骨骼活化时间延长，导致2周后更广泛的骨-植入物接触。总之，ASD表面处理增强了体外的生物反应，促进了早期成骨细胞的粘附，并增强了体内的骨整合特性，从而加速了主要的成骨反应。

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