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首页> 外文期刊>Acta biomaterialia >Transplantation of human mesenchymal stem cells in a non-autogenous setting for bone regeneration in a rabbit critical-size defect model.
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Transplantation of human mesenchymal stem cells in a non-autogenous setting for bone regeneration in a rabbit critical-size defect model.

机译：非自体环境中人间充质干细胞的移植，用于兔临界尺寸缺损模型中的骨再生。

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摘要

Human mesenchymal stem cells (hMSC) represent an attractive cell population for tissue engineering purposes. Furthermore, hMSC are described as immune privileged, and non-autogenous application seems possible. The current study examines the regeneration potential of hMSC after xenogenic transplantation compared with autogenous rabbit MSC in a critical-size bone defect. After isolation, hMSC and rabbit MSC were seeded on calcium-deficient hydroxyapatite (CDHA) and transplanted into a radial critical-size defect of New Zealand white rabbits. Defects were filled with a CDHA scaffold seeded with autogenous rabbit MSC, CDHA seeded with xenogenic hMSC or unseeded CDHA. An empty defect served as control group. Animals were sacrificed after 3 months. Evaluation was performed using radiography, micro-computed tomography (micro-CT) and histology. In addition, a non-destructive four-point-bending test was performed in order to evaluate biomechanical stiffness. While autogenous MSC seeded on CDHA led to increased healing of critical-size bone defects from radiological (micro-CT; p = 0.009) and histological (p = 0.048) perspectives compared with unloaded CDHA, it was not possible to demonstrate analogous effects for the xenogenic transplantation of hMSC. The xenogenic treatment group displayed inferior results in all parameters compared with the autogenous MSC treatment group (histology p = 0.041; micro-CT p = 0.006; biomechanical testing p = 0.017). Nevertheless, no local or systemic inflammatory response resulting from xenogenic transplantation was observed. While previous papers suggest the use of non-autogenous hMSC cells for tissue engineering purposes, the present results show inferior clinical results from transplantation of hMSC in a xenogenic setting compared with autogenous MSC.
机译：人间充质干细胞（hMSC）代表用于组织工程目的的有吸引力的细胞群。此外，hMSC被描述为具有免疫特权，并且非自体应用似乎是可能的。目前的研究检查了异种移植后hMSC与自体兔MSC在临界大小骨缺损中的再生潜力。分离后，将hMSC和兔MSC接种在缺钙的羟基磷灰石（CDHA）上，并移植到新西兰白兔的放射状临界大小缺损中。缺陷中填充有接种自体兔MSC的CDHA支架，接种异种hMSC或未接种CDHA的CDHA。空缺损作为对照组。 3个月后处死动物。使用放射线照相，微型计算机断层扫描（micro-CT）和组织学进行评估。此外，进行了无损四点弯曲测试，以评估生物力学刚度。从放射学（micro-CT; p = 0.009）和组织学（p = 0.048）角度来看，植入CDHA的自体MSC导致临界尺寸骨缺损的愈合与未装载CDHA相比有所提高，但无法证明对hMSC的异种移植。与自体MSC治疗组相比，异种治疗组在所有参数上均显示较差的结果（组织学p = 0.041;微型CT p = 0.006;生物力学测试p = 0.017）。然而，未观察到异种移植引起的局部或全身炎症反应。尽管先前的论文建议将非自体hMSC细胞用于组织工程目的，但本研究结果显示，与自体MSC相比，异种环境下hMSC的移植临床效果较差。

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