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首页> 外文期刊>Acta biomaterialia >Influence of cell-adhesive peptide ligands on poly(ethylene glycol) hydrogel physical, mechanical and transport properties.
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Influence of cell-adhesive peptide ligands on poly(ethylene glycol) hydrogel physical, mechanical and transport properties.

机译：细胞粘附肽配体对聚乙二醇水凝胶物理，机械和运输性能的影响。

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摘要

Synthetic three-dimensional scaffolds for cell and tissue engineering routinely utilize peptide ligands to provide sites for cell adhesion and to promote cellular activity. Given the fact that recent studies have dedicated great attention to the mechanisms by which cell behavior is influenced by various ligands and scaffold material properties, it is surprising that little work to date has been carried out to investigate the influence of covalently bound ligands on hydrogel material properties. Herein we report the influence of three common ligands utilized in tissue engineering, namely RGD, YIGSR and IKVAV, on the mechanical properties of cross-linked poly(ethylene glycol) (PEG) hydrogels. The effect of the ligands on hydrogel storage modulus, swelling ratio, mesh size and also on the diffusivity of bovine serum albumin through the hydrogel were investigated in detail. We identified conditions under which these ligands strikingly influence the properties of the material. The extent of influence and whether the ligand increases or decreases a specific property is linked to ligand type and concentration. Further, we pinpoint mechanisms by which the ligands interact with the PEG network. This work thus provides specific evidence for interactions between peptide ligands and cross-linked PEG hydrogels that have a significant impact on hydrogel material and transport properties. As a result, this work may have important implications for interpreting cell experiments carried out with ligand-modified hydrogels, because the addition of ligand may affect not only the scaffold's biological properties, but also key physical properties of the system.
机译：用于细胞和组织工程的合成三维支架通常利用肽配体来提供细胞粘附位点并促进细胞活性。鉴于最近的研究非常关注细胞行为受各种配体和支架材料特性影响的机制这一事实，令人惊讶的是，迄今为止，几乎没有开展研究共价结合配体对水凝胶材料的影响的工作属性。本文中，我们报道了在组织工程中使用的三种常见配体，即RGD，YIGSR和IKVAV，对交联聚乙二醇（PEG）水凝胶的机械性能的影响。详细研究了配体对水凝胶储能模量，溶胀率，筛孔尺寸以及牛血清白蛋白通过水凝胶扩散性的影响。我们确定了这些配体显着影响材料性能的条件。影响程度以及配体是增加还是减少特定性质都与配体类型和浓度有关。此外，我们查明了配体与PEG网络相互作用的机制。因此，这项工作为肽配体与交联的PEG水凝胶之间的相互作用提供了特殊的证据，这些相互作用对水​​凝胶的材料和运输特性产生了重大影响。结果，这项工作可能对解释用配体修饰的水凝胶进行的细胞实验具有重要意义，因为配体的添加不仅会影响支架的生物学特性，而且还会影响系统的关键物理特性。

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