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首页> 外文期刊>Acta biomaterialia >Effects of phosphoric acid treatment of titanium surfaces on surface properties, osteoblast response and removal of torque forces.
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Effects of phosphoric acid treatment of titanium surfaces on surface properties, osteoblast response and removal of torque forces.

机译：磷酸处理钛表面对表面性能，成骨细胞反应和扭矩去除的影响。

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摘要

This study investigated the surface characteristics and biocompatibility of phosphate ion (P)-incorporated titanium (Ti) surfaces hydrothermally treated with various concentrations of phosphoric acid (H(3)PO(4)). The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, X-ray photoelectron spectroscopy, optical profilometry, contact angle and surface energy measurement and inductively coupled plasma mass spectroscopy (ICP-MS). MC3T3-E1 cell attachment, spreading, proliferation and osteoblastic gene expression on different surfaces were evaluated. The degree of bony integration was biomechanically evaluated by removal torque testing after 4 weeks of healing in rabbit tibiae. The H(3)PO(4) treatment produced micro-rough Ti surfaces with crystalline P-incorporated Ti oxide layers. High concentration H(3)PO(4) treatment (1% and 2%) produced significantly higher hydrophilic surfaces compared with low H(3)PO(4) treatment (0.5%) and untreated surfaces (P<0.01). ICP-MS analysis showed P ions were released from P-incorporated surfaces. Significant increased cell attachment (P<0.05) and notably higher mRNA expressions of Runx2, alkaline phosphatase, osteopontin and osteocalcin were observed in cells grown on P-incorporated surfaces compared with cells on untreated machined surfaces. P-incorporated surfaces showed significantly higher removal torque forces compared with untreated machined implants (P<0.05). Ti surfaces treated with 2% H(3)PO(4) showed increasing tendencies in osteoblastic gene expression and removal torque forces compared with those treated with lower H(3)PO(4) concentrations or untreated surfaces. These results demonstrate that H(3)PO(4) treatment may improve the biocompatibility of Ti implants by enhancing osteoblast attachment, differentiation and biomechanical anchorage.
机译：这项研究调查了表面特征和生物相容性的磷酸根离子（P）结合钛（Ti）表面用不同浓度的磷酸（H（3）PO（4））进行水热处理。通过扫描电子显微镜，薄膜X射线衍射法，X射线光电子能谱，光学轮廓，接触角和表面能测量以及电感耦合等离子体质谱（ICP-MS）来评估表面特性。评价了MC3T3-E1在不同表面上的细胞附着，扩散，增殖和成骨细胞基因表达。兔胫骨愈合4周后，通过去除扭矩测试对骨整合程度进行生物力学评估。 H（3）PO（4）处理产生微粗糙的Ti表面，并带有结晶P结合的Ti氧化物层。高浓度H（3）PO（4）处理（1％和2％）产生的亲水性表面明显高于低H（3）PO（4）处理（0.5％）和未处理的表面（P <0.01）。 ICP-MS分析表明，P离子从结合P的表面释放出来。与未处理的加工表面相比，在掺有P的表面上生长的细胞中细胞附着明显增加（P <0.05），并且Runx2，碱性磷酸酶，骨桥蛋白和骨钙素的mRNA表达明显更高。与未处理的机加工植入物相比，掺有P的表面显示出更高的去除扭矩力（P <0.05）。与2％H（3）PO（4）浓度或未处理表面相比，用2％H（3）PO（4）处理的Ti表面显示出成骨细胞基因表达和去除扭矩力增加的趋势。这些结果表明，H（3）PO（4）处理可以通过增强成骨细胞附着，分化和生物力学锚固来改善Ti植入物的生物相容性。

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